US 11,796,547 B2
Methods of detecting donor-specific antibodies and systems for practicing the same
Dolly B. Tyan, Palo Alto, CA (US); and Ge Chen, Redwood City, CA (US)
Assigned to The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed by The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed on Aug. 17, 2020, as Appl. No. 16/995,614.
Application 16/995,614 is a continuation of application No. 16/109,481, filed on Aug. 22, 2018, granted, now 10,746,744.
Application 16/109,481 is a continuation of application No. 14/775,150, granted, now 10,101,337, issued on Oct. 16, 2018, previously published as PCT/US2014/026406, filed on Mar. 13, 2014.
Claims priority of provisional application 61/782,003, filed on Mar. 14, 2013.
Prior Publication US 2021/0199662 A1, Jul. 1, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/68 (2006.01); G01N 33/543 (2006.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01); G01N 33/58 (2006.01)
CPC G01N 33/686 (2013.01) [G01N 33/5091 (2013.01); G01N 33/5094 (2013.01); G01N 33/54313 (2013.01); G01N 33/54333 (2013.01); G01N 33/56977 (2013.01); G01N 33/582 (2013.01); G01N 33/6854 (2013.01); G01N 2333/4716 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A kit for use in a method for determining the presence or absence of donor-specific antibodies in a biological sample from a recipient, the kit comprising:
a plurality of beads comprising capture antibodies attached to a surface thereof that specifically bind a donor human leukocyte antigen (HLA) in a complex comprising the donor HLA bound to a recipient immune antibody;
a lysis buffer; and
a detectably-labelled antibody, wherein the detectably-labelled antibody is capable of specifically binding the recipient immune antibody in complex with the donor HLA under lysis conditions.