CPC G01N 33/686 (2013.01) [G01N 33/5091 (2013.01); G01N 33/5094 (2013.01); G01N 33/54313 (2013.01); G01N 33/54333 (2013.01); G01N 33/56977 (2013.01); G01N 33/582 (2013.01); G01N 33/6854 (2013.01); G01N 2333/4716 (2013.01)] | 15 Claims |
1. A kit for use in a method for determining the presence or absence of donor-specific antibodies in a biological sample from a recipient, the kit comprising:
a plurality of beads comprising capture antibodies attached to a surface thereof that specifically bind a donor human leukocyte antigen (HLA) in a complex comprising the donor HLA bound to a recipient immune antibody;
a lysis buffer; and
a detectably-labelled antibody, wherein the detectably-labelled antibody is capable of specifically binding the recipient immune antibody in complex with the donor HLA under lysis conditions.
|