US 11,796,499 B2
Immobilization strategies for enhancing sensitivity of electrochemical aptamer-based sensors
Yi Xiao, Cary, NC (US); Yingzhu Liu, Wuhan (CN); and Juan Canoura, Hialeah Gardens, FL (US)
Assigned to THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES, Miami, FL (US)
Filed by Yi Xiao, Cary, NC (US); Yingzhu Liu, Wuhan (CN); and Juan Canoura, Hialeah Gardens, FL (US)
Filed on Dec. 13, 2022, as Appl. No. 18/65,118.
Claims priority of provisional application 63/288,882, filed on Dec. 13, 2021.
Prior Publication US 2023/0213471 A1, Jul. 6, 2023
Int. Cl. G01N 27/327 (2006.01); G01N 33/543 (2006.01); G01N 33/53 (2006.01)
CPC G01N 27/3276 (2013.01) [G01N 27/3277 (2013.01); G01N 33/5308 (2013.01); G01N 33/5438 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method for fabricating an electrochemical aptamer-based (E-AB) sensor, the method comprising:
providing an electrode;
incubating the electrode with an immobilizing solution comprising aptamer-target complexes each comprising an aptamer bound to an analyte, the immobilizing solution having a low ionic strength;
contacting the electrode with a solution comprising a backfiller; and
removing the target from each of the aptamer-target complexes.