	US 11,795,485 B2
	Selective enrichment of a population of DNA in a mixed DNA sample through targeted suppression of DNA amplification
Chiahao Tsui, Somerville, MA (US); Melis Nuray Anahtar, Cambridge, MA (US); Dougal Maclaurin, Cambridge, MA (US); Miriam H. Huntley, Cambridge, MA (US); and Jeffrey D. Brewster, Glenside, PA (US)
Assigned to Day Zero Diagnostics, Inc., Boston, MA (US)
Appl. No. 16/757,314
Filed by Day Zero Diagnostics, Inc., Allston, MA (US)
PCT Filed Oct. 18, 2018, PCT No. PCT/US2018/056598 § 371(c)(1), (2) Date Apr. 17, 2020, PCT Pub. No. WO2019/079656, PCT Pub. Date Apr. 25, 2019.
Claims priority of provisional application 62/573,720, filed on Oct. 18, 2017.
Prior Publication US 2020/0299741 A1, Sep. 24, 2020
Int. Cl. C12N 15/11 (2006.01); C12Q 1/68 (2018.01); C12P 19/34 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/689 (2018.01)

CPC C12P 19/34 (2013.01) [C12N 15/11 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/689 (2013.01)]

20 Claims

1. An improved method for enrichment of a population of DNA by amplification of a mixed sample of DNA comprising a first population and a second population, the improvement comprising:

(a) contacting the mixed sample of DNA with a plurality of blocking oligonucleotides which specifically bind to a plurality of oligonucleotide sequences in the first population of DNA; and

(b) subjecting said mixed sample of DNA to whole genome amplification (WGA) to produce an amplified mixed sample of DNA;

wherein said blocking oligonucleotides suppress amplification of the first population of DNA and thereby enrich the second population of DNA relative to the first population of DNA in the amplified mixed sample.