CPC C12N 15/902 (2013.01) [C12N 9/22 (2013.01); C12N 15/102 (2013.01); C12N 15/1024 (2013.01); C12N 15/1082 (2013.01); C12N 15/1093 (2013.01); C12N 15/11 (2013.01); C12N 15/111 (2013.01); C40B 40/08 (2013.01); C12N 2310/10 (2013.01); C12N 2310/20 (2017.05); C12N 2320/11 (2013.01); C12N 2320/12 (2013.01); C12N 2330/31 (2013.01)] | 20 Claims |
1. A vector for editing a cell created by:
ligating into the vector that comprises a first region of a gRNA a second nucleotide sequence comprising:
a region homologous to a target region in a cell comprising a change in sequence relative to the target region;
a site conferring immunity to nuclease-mediated editing; and
a nucleic acid sequence comprising a second region of the gRNA, whereupon the ligation forms a sequence within the vector comprising a functional gRNA;
thus generating the vector for editing the cell.
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