US 11,795,432 B2
Passive replacement of media
Brian J. Nankervis, Golden, CO (US)
Assigned to Terumo BCT, Inc., Lakewood, CO (US)
Filed by Terumo BCT, Inc., Lakewood, CO (US)
Filed on Dec. 20, 2017, as Appl. No. 15/849,309.
Application 15/849,309 is a division of application No. 14/668,659, filed on Mar. 25, 2015, granted, now 11,008,547.
Claims priority of provisional application 61/970,274, filed on Mar. 25, 2014.
Prior Publication US 2018/0119094 A1, May 3, 2018
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/071 (2010.01); C12M 1/34 (2006.01); C12M 1/12 (2006.01); C12M 1/00 (2006.01); C12M 1/36 (2006.01); C12M 1/42 (2006.01)
CPC C12N 5/0602 (2013.01) [C12M 25/10 (2013.01); C12M 29/00 (2013.01); C12M 29/16 (2013.01); C12M 29/26 (2013.01); C12M 35/08 (2013.01); C12M 41/00 (2013.01); C12M 41/48 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A cell expansion system, comprising:
a bioreactor comprising a first fluid flow path having at least opposing ends, a first opposing end of the first fluid flow path fluidly associated with a first port of a hollow fiber membrane and a second end of the first fluid flow path fluidly associated with a second port of the hollow fiber membrane, wherein the first fluid flow path comprises an intracapillary portion of the hollow fiber membrane;
a fluid inlet path fluidly associated with the first fluid flow path, wherein a plurality of cells are introduced into the first fluid flow path through the first fluid inlet path;
a first pump that circulates fluid in the first fluid flow path of the bioreactor;
an outlet line in fluid communication with the bioreactor, wherein the outlet line is attached, at a first time, to an outlet bag in the cell expansion system; and
a controller that controls operation of the first pump, wherein the controller is configured to control the first pump to:
circulate a fluid at a first rate within the first fluid flow path; and
stop, when the outlet bag is replaced with a media bag at a second time, the circulation of the fluid within the first fluid flow path after the plurality of the cells are loaded into the bioreactor, wherein the outlet line is attached to the media bag at the second time; and
wherein media from the media bag is added to the bioreactor while the circulation of the fluid within the first fluid flow path is stopped.