US 11,795,423 B2
Micro-fabricated multi-well chips for long term automated imaging of C. elegans growth and behavior
Christopher Fang-Yen, Philadelphia, PA (US)
Assigned to The Trustees of the University of Pennsylvania, Philadelphia, PA (US)
Appl. No. 15/318,059
Filed by THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA, Philadelphia, PA (US)
PCT Filed Jun. 26, 2015, PCT No. PCT/US2015/037997
§ 371(c)(1), (2) Date Dec. 12, 2016,
PCT Pub. No. WO2015/200803, PCT Pub. Date Dec. 30, 2015.
Claims priority of provisional application 62/017,579, filed on Jun. 26, 2014.
Prior Publication US 2017/0107470 A1, Apr. 20, 2017
Int. Cl. B01L 3/00 (2006.01); C12M 1/32 (2006.01); C12M 1/00 (2006.01); C12N 5/00 (2006.01)
CPC C12M 23/12 (2013.01) [B01L 3/5085 (2013.01); B01L 3/50857 (2013.01); C12M 23/22 (2013.01); C12N 5/0068 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0858 (2013.01); B01L 2300/10 (2013.01); C12N 2533/00 (2013.01); C12N 2535/10 (2013.01)] 11 Claims
OG exemplary drawing
 
1. An apparatus, comprising:
a substrate having a planar array of conical-shaped depressions, wherein each depression is defined by an upper opening, a concave side wall and a bottom surface,
wherein the substrate further comprises a network of channels that are arranged in a grid-like structure and are interconnected to form a continuous path through the substrate, wherein the channels are disposed around each depression of said array of depressions to form a dedicated moat for each depression; and
a tray, wherein said substrate is configured to be inserted into said tray.