US 11,795,240 B2
Enhancing activity of CAR T cells by co-introducing a bispecific antibody
Carl H. June, Merion Station, PA (US); Yangbing Zhao, Lumberton, NJ (US); and Xiaojun Liu, Wallingford, PA (US)
Assigned to The Trustees of the University of Pennsylvania, Philadelphia, PA (US)
Filed by The Trustees of the University of Pennsylvania, Philadelphia, PA (US)
Filed on Jun. 24, 2020, as Appl. No. 16/910,837.
Application 15/690,383 is a division of application No. 14/410,427, granted, now 9,765,156, previously published as PCT/US2013/050275, filed on Jul. 12, 2013.
Application 16/910,837 is a continuation of application No. 15/690,383, filed on Aug. 30, 2017, granted, now 10,696,749.
Claims priority of provisional application 61/671,535, filed on Jul. 13, 2012.
Prior Publication US 2021/0040234 A1, Feb. 11, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 16/16 (2006.01); C07K 16/28 (2006.01); A61K 35/17 (2015.01); A61K 47/68 (2017.01); C07K 16/46 (2006.01); A61K 39/00 (2006.01); C12N 5/16 (2006.01); C12N 15/62 (2006.01)
CPC C07K 16/468 (2013.01) [A61K 35/17 (2013.01); A61K 47/6879 (2017.08); C07K 16/2803 (2013.01); C07K 16/2809 (2013.01); A61K 2039/505 (2013.01); C07K 2317/21 (2013.01); C07K 2317/24 (2013.01); C07K 2317/31 (2013.01); C07K 2317/622 (2013.01); C07K 2319/00 (2013.01); C12N 5/16 (2013.01); C12N 15/62 (2013.01); C12N 2510/00 (2013.01)] 16 Claims
 
1. An ex vivo or in vitro method of generating a modified T cell, comprising introducing into a T cell a composition comprising
(a) a first RNA molecule encoding a chimeric antigen receptor (CAR), wherein the CAR comprises an antigen binding domain, a transmembrane domain, and a CD3 zeta signaling domain; and
(b) a second RNA molecule encoding a bispecific antibody, wherein the bispecific antibody comprises a first antigen recognition domain that recognizes the same antigen as the antigen binding domain of the CAR, and a second antigen recognition domain that recognizes CD3 wherein the bispecific antibody encoded by the second RNA molecule is secreted by the cell.