CPC C07K 16/1009 (2013.01) [C07K 7/06 (2013.01); C07K 14/70503 (2013.01); C07K 16/2803 (2013.01); C07K 16/2818 (2013.01); C12N 15/1138 (2013.01); G01N 33/502 (2013.01); G01N 33/505 (2013.01); G01N 33/5011 (2013.01); G01N 33/574 (2013.01); A61K 2039/507 (2013.01); C07K 2317/74 (2013.01); C07K 2319/30 (2013.01); C12N 2310/14 (2013.01); G01N 2333/70596 (2013.01); G01N 2500/02 (2013.01); G01N 2500/10 (2013.01); Y02A 50/30 (2018.01)] | 15 Claims |
1. A method of screening for anti-PVRIG antibodies and fragments thereof, wherein the method comprises:
i) providing a first cell comprising an exogenous recombinant nucleic acid encoding a human PVRIG polypeptide, wherein the first cell expresses the human PVRIG polypeptide;
ii) contacting the first cell with a second cell comprising another exogenous recombinant nucleic acid encoding a human PVRL2 polypeptide, wherein the second cell expresses the human PVRL2 polypeptide, in the presence of a candidate agent comprising an antibody or fragment thereof; and
iii) determining whether the first cell binds to the second cell as an indication of whether the candidate agent comprising an antibody or fragment thereof comprises an anti-PVRIG antibody or a fragment thereof that inhibits the binding of PVRIG with PVRL2.
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