US 11,793,840 B2
Nano-armored single cell product for treating Parkinson's disease through bacteria and a preparation method thereof
Bin Zheng, Shenzhen (CN); Yulin Cao, Shenzhen (CN); Wei Sun, Shenzhen (CN); Qinglu Guo, Shenzhen (CN); and Shixiang Cheng, Shenzhen (CN)
Assigned to Tangyi Holdings(Shenzhen) Limited, Shenzhen (CN); and Healthina Stem Cell Industry Platform (Tianjin) Limited, Tianjin (CN)
Filed by Tangyi Holdings(Shenzhen) Limited, Shenzhen (CN); and Healthina Stem Cell Industry Platform (Tianjin) Limited, Tianjin (CN)
Filed on Oct. 18, 2021, as Appl. No. 17/503,561.
Claims priority of application No. 202110950540.6 (CN), filed on Aug. 18, 2021.
Prior Publication US 2023/0065910 A1, Mar. 2, 2023
Int. Cl. A61K 35/745 (2015.01); A61K 9/127 (2006.01); A61K 35/00 (2006.01)
CPC A61K 35/745 (2013.01) [A61K 9/127 (2013.01); A61K 9/1277 (2013.01); A61K 2035/115 (2013.01)] 1 Claim
 
1. A nano-armored single cell product, comprising:
a liposome coated Bifidobacterium LCB comprising a liposome and probiotics encapsulated by the liposome,
wherein the probiotics ferment to produce gamma-aminobutyric acid (GABA) that alleviates the activation of an inflammatory response in substantia nigra in a Parkinson's disease (PD) model, thus mitigating an inflammatory injury to dopaminergic neurons in substantia nigra and having a neuroprotective effect; whereby encapsulation of the probiotics by the liposome can protect the probiotics from strong acids and digestive enzymes in gastric acid;
wherein the probiotics producing GABA are Bifidobacterium adolescentis;
wherein the liposome comprises soy lecithin, cholesterol and vitamin E;
wherein a preparation method of nano-armored single cell product comprises the following steps:
S1) Preparation of liposome: taking soy lecithin, cholesterol and vitamin E at a ratio of 4:2:1, putting them in a chloroform solvent, and stirring the solution at room temperature until they are completely dissolved;
transferring the solution into a round-bottom flask, removing chloroform by reduction vaporization, adding a phosphate buffered solution into the flask, and filtering the solution with a microporous membrane after ultrasound to obtain a blank liposome;
dissolving an appropriate amount of Nile Red in the chloroform and mixing with the blank liposome to generate a Nile Red labeled liposome;
S2) Preparation of LCB: centrifugalizing 2 mL of a subculture of Bifidobacterium adolescentis, washing at a speed of 3,000 rpm, and dissolving precipitate in 1 mL of Nile Red labeled liposome solution; mixing the synthetic liposome and the bacterium in a calcium chloride solution for 15 min to synthesize the LCB; and wherein
the nano-armored single cell product obtained is used in drugs for treating symptoms of PD in animal bodies.