US 11,793,431 B2
Systems and methods for the detection and quantification of ammonia and ammonium in fluids
Marylaura Thomas, Scottsdale, AZ (US); Leslie Thomas, Scottsdale, AZ (US); and Erica Forzani, Scottsdale, AZ (US)
Assigned to Mayo Foundation for Medical Education and Research, Rochester, MN (US); and Arizona Board of Regents on behalf of Arizona State University, Scottsdale, AZ (US)
Appl. No. 16/959,551
Filed by Mayo Foundation for Medical Education and Research, Rochester, MN (US); and Arizona Board of Regents on behalf of Arizona State University, Scottsdale, AZ (US)
PCT Filed Jan. 11, 2019, PCT No. PCT/US2019/013237
§ 371(c)(1), (2) Date Jul. 1, 2020,
PCT Pub. No. WO2019/140224, PCT Pub. Date Jul. 18, 2019.
Claims priority of provisional application 62/617,053, filed on Jan. 12, 2018.
Prior Publication US 2021/0076999 A1, Mar. 18, 2021
Int. Cl. A61B 5/1455 (2006.01); A61B 5/145 (2006.01); A61B 5/20 (2006.01); A61B 5/00 (2006.01); B01D 53/22 (2006.01)
CPC A61B 5/14552 (2013.01) [A61B 5/14546 (2013.01); A61B 5/201 (2013.01); A61B 5/4866 (2013.01); B01D 53/228 (2013.01); B01D 2259/10 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A system comprising an analyzer device configured to be in fluid communication with a sample of a bodily fluid, the analyzer device comprising:
a gas sensing chamber;
an extraction membrane located between: (i) an area in fluid communication with the bodily fluid and (ii) the gas sensing chamber, wherein the extraction membrane is configured to: (a) extract and convert chemically or electrochemically at least a portion of ammonium (NH4+) contained within the bodily fluid into ammonia (NH3), (b) eliminate interferences, and (c) dispel the converted ammonia (NH3) into the gas sensing chamber; and
an ammonia (NH3) sensor located within the gas sensing chamber, the ammonia sensor comprising a processor with a non-transitory memory storing instructions that, when executed, causes the processor to quantify an amount of ammonia (NH3) present in the gas sensing chamber in relation to a total ammonia of the bodily fluid, wherein the total ammonia is a sum of the ammonia (NH3) and ammonium (NH4+),
wherein the analyzer device detects, based on the quantified amount of ammonia present in the gas sensing chamber, at least one of: altered organ function, altered tissue function, and altered metabolic status.