US 11,793,202 B2
Methods of use of seed-origin endophyte populations
Geoffrey von Maltzahn, Boston, MA (US); Richard Bailey Flavell, Thousand Oaks, CA (US); Gerardo V. Toledo, Belmont, MA (US); Slavica Djonovic, Malden, MA (US); Luis Miguel Marquez, Belmont, MA (US); David Morris Johnston, Cambridge, MA (US); Yves Alain Millet, Newtonville, MA (US); Jeffrey Lyford, Hollis, NH (US); Alexander Naydich, Cambridge, MA (US); and Craig Sadowski, Somerville, MA (US)
Assigned to Indigo Ag, Inc., Boston, MA (US)
Filed by Indigo Ag, Inc., Boston, MA (US)
Filed on Oct. 4, 2021, as Appl. No. 17/493,779.
Application 17/493,779 is a continuation of application No. 16/657,929, filed on Oct. 18, 2019, granted, now 11,166,465.
Application 16/657,929 is a continuation of application No. 16/024,029, filed on Jun. 29, 2018, granted, now 10,499,652, issued on Dec. 10, 2019.
Application 16/024,029 is a continuation of application No. 15/436,592, filed on Feb. 17, 2017, granted, now 10,058,101, issued on Aug. 28, 2018.
Application 15/436,592 is a continuation of application No. 15/145,687, filed on May 3, 2016, granted, now 9,622,485, issued on Apr. 18, 2017.
Application 15/145,687 is a continuation of application No. 15/017,531, filed on Feb. 5, 2016, granted, now 9,532,573, issued on Jan. 3, 2017.
Application 15/017,531 is a continuation of application No. 14/704,891, filed on May 5, 2015, granted, now 9,288,995, issued on Mar. 22, 2016.
Application 14/704,891 is a continuation of application No. 14/316,469, filed on Jun. 26, 2014, granted, now 9,113,636, issued on Aug. 25, 2015.
Claims priority of provisional application 61/935,761, filed on Feb. 4, 2014.
Claims priority of provisional application 61/959,859, filed on Sep. 4, 2013.
Claims priority of provisional application 61/959,847, filed on Sep. 4, 2013.
Claims priority of provisional application 61/959,854, filed on Sep. 4, 2013.
Claims priority of provisional application 61/959,861, filed on Sep. 4, 2013.
Claims priority of provisional application 61/959,858, filed on Sep. 4, 2013.
Claims priority of provisional application 61/959,870, filed on Sep. 4, 2013.
Claims priority of provisional application 61/957,255, filed on Jun. 26, 2013.
Prior Publication US 2022/0232833 A1, Jul. 28, 2022
Int. Cl. A01N 63/20 (2020.01); A01C 1/06 (2006.01); G01N 33/50 (2006.01); A01C 1/00 (2006.01)
CPC A01N 63/20 (2020.01) [A01C 1/06 (2013.01); G01N 33/5097 (2013.01); A01C 1/00 (2013.01)] 18 Claims
 
1. A method of treating a cereal agricultural plant, comprising mechanically or manually contacting a seed of a cereal agricultural plant with an agricultural formulation comprising an agriculturally acceptable carrier and a purified bacterial population, wherein the purified bacterial population comprises a Bacillus bacterial endophyte that is heterologous to the seed, has a 16S nucleic acid sequence that is at least 97% identical to a 16S nucleic acid sequence selected from the group consisting of SEQ ID NO: 542, 617, 629, 745, and 746, wherein the purified bacterial population is present in the formulation in an amount capable of increasing shoot biomass of an agricultural plant derived from the seed under low nutrient stress.