US 12,449,647 B2
Light sheet microscope and method for manipulating a target area of a sample
Christian Schumann, Lich (DE)
Assigned to LEICA MICROSYSTEMS CMS GMBH, Wetzlar (DE)
Appl. No. 17/915,111
Filed by LEICA MICROSYSTEMS CMS GMBH, Wetzlar (DE)
PCT Filed Mar. 31, 2020, PCT No. PCT/EP2020/059130
§ 371(c)(1), (2) Date Sep. 28, 2022,
PCT Pub. No. WO2021/197587, PCT Pub. Date Oct. 7, 2021.
Prior Publication US 2023/0152562 A1, May 18, 2023
Int. Cl. G02B 21/00 (2006.01)
CPC G02B 21/0032 (2013.01) [G02B 21/0044 (2013.01); G02B 21/008 (2013.01)] 16 Claims
OG exemplary drawing
 
13. A light sheet microscope, comprising:
a light source configured to emit illumination light;
a manipulation light source configured to emit manipulation light for manipulating a target area of a sample;
an optical system configured to form a light sheet from the illumination light in a sample space, the light sheet having a width in a width direction, the width direction being perpendicular to both a light propagation direction and a thickness direction;
wherein the optical system has an optical transport system configured to transport an intermediate image of the light sheet from an intermediate image space into the sample space;
an optical detection system configured to detect detection light emitted by the sample; and
an optical erecting unit comprising a first objective and a second objective facing the intermediate image space, the first objective being configured to direct the manipulation light into the optical transport system, and the second objective being configured to direct the detection light into the optical detection system,
wherein the optical detection system comprises a light deflection element configured to direct the manipulation light into the second objective, and
wherein optical axes of the first objective and the second objective are oblique to each other, so that the target area is tilted with respect to the width direction of the light sheet.