US 12,449,438 B2
Cell picking device and cell picking method
Toru Ezure, Kyoto (JP); Mika Okada, Kyoto (JP); and Yuki Kuzuhara, Kyoto (JP)
Assigned to SHIMADZU CORPORATION, Kyoto (JP)
Filed by SHIMADZU CORPORATION, Kyoto (JP)
Filed on Nov. 23, 2021, as Appl. No. 17/533,850.
Claims priority of application No. 2020-202967 (JP), filed on Dec. 7, 2020.
Prior Publication US 2022/0178961 A1, Jun. 9, 2022
Int. Cl. G01N 35/10 (2006.01); B01L 3/02 (2006.01); B01L 9/00 (2006.01); C12M 1/00 (2006.01); C12M 1/26 (2006.01); C12N 1/02 (2006.01)
CPC G01N 35/1011 (2013.01) [B01L 3/021 (2013.01); B01L 9/54 (2013.01); C12M 33/04 (2013.01); C12M 47/02 (2013.01); C12N 1/02 (2013.01); B01L 2300/027 (2013.01); G01N 2035/103 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A method of cell picking for sucking cells from a liquid sample in a sample container with a cell picking device, the cell picking device comprising:
a sucking member to which a pipette tip is attachable;
a driver that moves the sucking member and performs suction through the sucking member and the pipette tip;
a work mode switcher, comprising a processor and memory, which is configured to switch a work mode of the driver between an adherent cell mode and a suspension cell mode; and
a controller that controls the driver;
the method comprising:
switching the work mode between the adherent cell mode and the suspension cell mode by the work mode switcher;
performing first advancing work for bringing an end of the pipette tip into contact with a bottom surface in the sample container by causing the sucking member to advance in an axial direction of the pipette tip with the pipette tip, attached to the sucking member, being tilted with respect to a vertical direction, scanning work for scanning the bottom surface of the sample container toward a first position using the end of the pipette tip by moving the sucking member in a horizontal direction, tilting work for lifting the end of the pipette tip and lowering a base of the pipette tip in the first position by tilting the sucking member further with respect to the vertical direction, and sucking work for sucking the sample in the sample container through the sucking member and the pipette tip from stripped cells adhering to the bottom surface in the sample container, in the adherent cell mode by the controller; and
performing second advancing work for causing the end of the pipette tip to advance to a second position farther upward than the first position in the sample container by causing the sucking member to advance in the axial direction of the pipette tip with the pipette tip, attached to the sucking member, being tilted with respect to the vertical direction, and the sucking work for sucking cells in suspension in the liquid sample with the end of the pipette tip located in the second position, in the suspension cell mode by the controller,
wherein the second position is located between the bottom surface in the sample container and a liquid surface in the sample container.