US 12,449,428 B2
On-demand electrochemical reactions in nano-electrospray ionization mass spectrometry for biomolecular analysis
Xin Yan, College Station, TX (US); Shuli Tang, College Station, TX (US); and Heyong Cheng, College Station, TX (US)
Assigned to The Texas A&M University System, College Station, TX (US)
Appl. No. 17/769,409
Filed by THE TEXAS A&M UNIVERSITY SYSTEM, College Station, TX (US)
PCT Filed Oct. 23, 2020, PCT No. PCT/US2020/057037
§ 371(c)(1), (2) Date Apr. 15, 2022,
PCT Pub. No. WO2021/081313, PCT Pub. Date Apr. 29, 2021.
Claims priority of provisional application 62/924,889, filed on Oct. 23, 2019.
Prior Publication US 2023/0333124 A1, Oct. 19, 2023
Int. Cl. G01N 33/92 (2006.01)
CPC G01N 33/92 (2013.01) [G01N 2560/00 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method for identifying a sn-position of a lipid, the method comprising:
conducting a first voltage-controlled electrochemical reaction that converts the lipid to a first derivatized lipid of the lipid, wherein the first voltage-controlled electrochemical reaction comprises producing in situ a Co2+ ion by anodic corrosion of an electrospray ionization (ESI) electrode and complexing the Co2+ ion to the lipid; and
fragmenting the first derivatized lipid to generate a first diagnostic ion, wherein the first diagnostic ion identifies the sn-position of the lipid.