	US 12,449,424 B2
	In vitro method for detecting avian intestinal dysbiosis
Stefan Pelzer, Gütersloh (DE); Monika Flügel, Steinhagen (DE); Sarah Hark, Gütersloh (DE); Evy Goossens, Wachtebeke (BE); Filip Van Immerseel, Eke (BE); Richard Ducatelle, Wortegem-Petegem (BE); Lieven Van Meulebroek, Zottegem (BE); and Lynn Vanhaecke, Balegem (BE)
Assigned to Evonik Operations GmbH, Essen (DE)
Appl. No. 17/413,548
Filed by EVONIK OPERATIONS GMBH, Essen (DE)
PCT Filed Dec. 9, 2019, PCT No. PCT/EP2019/084170 § 371(c)(1), (2) Date Jun. 12, 2021, PCT Pub. No. WO2020/120376, PCT Pub. Date Jun. 18, 2020.
Claims priority of application No. 18212599 (EP), filed on Dec. 14, 2018.
Prior Publication US 2022/0050115 A1, Feb. 17, 2022
Int. Cl. G01N 33/68 (2006.01); G01N 30/72 (2006.01)

CPC G01N 33/6848 (2013.01) [G01N 30/7233 (2013.01); G01N 33/6893 (2013.01); G01N 2800/06 (2013.01); G01N 2800/52 (2013.01)]

17 Claims

1. An in vitro method for detecting avian intestinal dysbiosis, comprising:

a) determining the presence or level of isoleucyl-arginine or isomers thereof in avian sample material, wherein the presence or an increased level of isoleucyl-arginine or isomers thereof in comparison to a non-affected control animal is indicative for avian intestinal dysbiosis, wherein the presence or level of isoleucyl-arginine or isomers thereof are detected or quantified via LC-MS, an enzyme assay, or LC combined with pre- or post-column derivatization and fluorescence or UV detection; and

b) administering probiotic agents, prebiotic agents, botanicals, organic/fatty acids, zeolites, bacteriophages or bacteriolytic enzymes or a combination thereof to animals exhibiting the presence of, or an increased level of, isoleucyl-arginine or isomers thereof in comparison to a control animal.