	US 12,449,416 B2
	Identification of health status in the elderly using immunological biomarkers
Jonathan Michael Blackburn, Singapore (SG); Arif Anwar, Kuala Lumpur (MY); Nurul H. Rutt, Singapore (SG); Anis Larbi, Singapore (SG); Olivier Nicolas Felix Cexus, Singapore (SG); Bernett Lee, Singapore (SG); Jesus Felix Bayta Valenzuela, Singapore (SG); Christopher Monterola, Singapore (SG); and Victor Tong, Singapore (SG)
Assigned to Sengenics Corporation Pte Ltd, (SG); and Agency for Science, Technology and Research, (SG)
Appl. No. 17/763,572
Filed by Sengenics Corporation Pte Ltd, Singapore (SG); and Agency for Science, Technology and Research, Singapore (SG)
PCT Filed Sep. 23, 2020, PCT No. PCT/SG2020/050540 § 371(c)(1), (2) Date Mar. 24, 2022, PCT Pub. No. WO2021/061048, PCT Pub. Date Apr. 1, 2021.
Claims priority of application No. 10201908922U (SG), filed on Sep. 25, 2019.
Prior Publication US 2023/0228741 A1, Jul. 20, 2023
Int. Cl. G01N 33/50 (2006.01); G01N 33/564 (2006.01); G01N 33/68 (2006.01)

CPC G01N 33/5091 (2013.01) [G01N 33/564 (2013.01); G01N 33/6875 (2013.01); G01N 2333/70596 (2013.01); G01N 2800/00 (2013.01)]

12 Claims

1. A method for manufacturing a kit for determining the health of a subject from a serum/plasma sample extracted from that subject, comprising the steps of:

for each antigen in a panel, cloning a biotin carboxyl carrier protein folding marker in-frame with a gene encoding the said antigen and expressing the resulting biotinylated antigen;

binding the biotinylated antigens to addressable locations on one or more streptavidin-coated substrates, thereby forming an antigen array;

such that the amount of autoantibodies from the sample binding to the antigens on the panel can be determined by exposing the substrate to the sample and measuring the response;

wherein the antigens comprise MAPK13 (HUMAN Mitogen-activated protein kinase kinase kinase 13, CD96 (HUMAN T-cell surface protein tactile), FKBP3 (HUMAN Peptidyl-prolyl cis-trans isomerase), PPM1A (HUMAN Protein phosphatase 1A), PHLDA1 (HUMAN Pleckstrin homology-like domain family A member 1), GLRX3 (HUMAN Glutaredoxin-3). FEN1 (HUMAN Flap endonuclease 1) and AURKA (HUMAN Aurora kinase A).