	US 12,449,415 B2
	Diagnostics
Gareth Ackland, London (GB)
Assigned to QUEEN MARY UNIVERSITY OF LONDON, London (GB)
Filed by Queen Mary University of London, London (GB)
Filed on Jul. 19, 2022, as Appl. No. 17/867,792.
Application 17/867,792 is a continuation of application No. 15/324,316, granted, now 11,415,573, previously published as PCT/GB2015/051986, filed on Jul. 8, 2015.
Claims priority of application No. 1412131 (GB), filed on Jul. 8, 2014.
Prior Publication US 2023/0043193 A1, Feb. 9, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/50 (2006.01); C12Q 1/00 (2006.01); C12Q 1/26 (2006.01); G01N 33/68 (2006.01)

CPC G01N 33/5047 (2013.01) [C12Q 1/005 (2013.01); C12Q 1/26 (2013.01); G01N 33/6812 (2013.01); G01N 2800/24 (2013.01); G01N 2800/26 (2013.01)]

19 Claims

1. A method of detecting infection or inflammation in a mammalian subject, the method comprising the steps of:

(a) detecting an amount of at least one marker selected from the group consisting of glutamate, lactate, ATP, D-serine, acetylcholine and adenosine in a sample from said subject,

(b) contacting the sample with a stimulator of immune cells, and

(c) detecting in the sample from step (b) an amount of the same marker(s) as detected in step (a), and determining whether the stimulus leads to a change in the amount of the said marker(s) detected in the sample as compared to the amount of marker(s) detected in step (a); and

(d) repeating steps (a) to (c) with a control sample, wherein an increase or decrease in the change of said marker(s) in the sample from the subject, when compared to the change of said marker(s) from the control sample, is indicative of the presence of inflammation or infection in the subject.