US 12,449,414 B2
Calibrating and normalizing an imager for optogenetics
Gabriel Benito Borja, Boston, MA (US); Steven Nagle, Cambridge, MA (US); Christopher Werley, Cambridge, MA (US); Yang Lu, Cambridge, MA (US); Adam Barnett, Cambridge, MA (US); Owen McManus, Belmont, MA (US); and Graham T. Dempsey, Cambridge, MA (US)
Assigned to QUIVER HOLDINGS INC., Cambridge, MA (US)
Filed by QUIVER HOLDINGS INC., Cambridge, MA (US)
Filed on Dec. 28, 2021, as Appl. No. 17/563,400.
Claims priority of provisional application 63/132,906, filed on Dec. 31, 2020.
Prior Publication US 2022/0205979 A1, Jun. 30, 2022
Int. Cl. G01N 33/483 (2006.01); G01N 33/487 (2006.01); G01N 33/50 (2006.01); G01N 33/542 (2006.01)
CPC G01N 33/5038 (2013.01) [G01N 33/48728 (2013.01); G01N 33/542 (2013.01)] 19 Claims
 
1. A method for assaying biological activity, the method comprising:
providing a sample comprising cells including optical reporters of cellular activity wherein the cells are in a well of a multi-well plate;
positioning the multi-well plate on a plate reader comprising a plurality of optical channels corresponding to a respective plurality of wells of the multi-well plate, wherein each optical channel is operable to provide to one well of the plurality of wells excitation and stimulation light of different wavelengths and to simultaneously read optical signals of different wavelengths from the one well;
stimulating, via one optical channel of the plurality of optical channels, the sample with a reference stimulus;
detecting by the one optical channel an optical signal produced in response to the reference stimulus;
exposing the sample to test conditions modeling a biological and/or chemical stimulus of the cellular activity;
detecting by the one optical channel an optical signal produced in response to the test conditions; and
calibrating the test signal to the reference signal to predict a level of activity of the cells in response to the modeled biological and/or chemical stimulus.