US 12,449,364 B2
Method and device for optically examining a biological sample
Nariman Ansari, Offenbach am Main (DE); and Falk Schlaudraff, Butzbach (DE)
Assigned to LEICA MICROSYSTEMS CMS GMBH, Wetzlar (DE)
Filed by Leica Microsystems CMS GmbH, Wetzlar (DE)
Filed on Jun. 21, 2021, as Appl. No. 17/352,401.
Claims priority of application No. 20182204 (EP), filed on Jun. 25, 2020.
Prior Publication US 2021/0404965 A1, Dec. 30, 2021
Int. Cl. G01N 21/64 (2006.01); G02B 21/00 (2006.01); G02B 21/36 (2006.01)
CPC G01N 21/6486 (2013.01) [G01N 21/6458 (2013.01); G02B 21/0032 (2013.01); G02B 21/0076 (2013.01); G02B 21/367 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A method for optically examining a biological sample, the method comprising:
preparing a light-activatable specimen including the biological sample, wherein the light-activatable specimen includes a medium containing light-sensitive envelopes in which molecules are encapsulated, the light-sensitive envelopes being configured to release the molecules when irradiated with activation light;
activating a target region of the light-activatable specimen by irradiating the activation light into the light-activatable specimen to release the molecules from the light-sensitive envelopes, the activation light being formed by a light sheet configured to illuminate a plane in the light-activatable specimen, the plane including at least a part of the biological sample; and
imaging the plane illuminated with the activation light of the light sheet that also activates the target region to release the molecules.