| CPC G01N 1/34 (2013.01) [G01N 1/4005 (2013.01); G01N 2001/4016 (2013.01)] | 14 Claims | 

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               1. A syringe column comprising: 
            a syringe barrel including a first portion and a second portion; 
                a solvated media bed including a first end, a second end and media selected from the group consisting of chromatographic media, extraction media, and enzymatic media, and disposed within the second portion of the syringe barrel; 
                a first frit disposed proximal to the second end of the solvated media bed; and 
                a pumping piston positioned in the syringe barrel proximal to the second end of the solvated media bed wherein the pumping piston comprises a piston stopper or a compression spring, and the piston stopper or the compression spring is configured to prevent the pumping piston from physically contacting the first end of the solvated media bed. 
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