| CPC C12Q 1/701 (2013.01) [C12Q 1/689 (2013.01); C12Q 1/6895 (2013.01); C12Q 1/705 (2013.01); C12Q 1/6818 (2013.01); C12Q 1/6851 (2013.01); C12Q 2600/16 (2013.01)] | 8 Claims |
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1. Primer and probe sets for pathogen detection of infection in a transplant patient, comprising the following 23 primer and probe sets, with each probe having a reporter group at a 5′ end and a quencher group at a 3′ end:
a 1st primer and probe set for detecting an adenovirus type B, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-TCAGGGATAACTTTGTAGG-3′ (SEQ ID NO: 1),
the downstream primer: 5′-GACAGTTCAGTATTTCTGTC-3′ (SEQ ID NO: 2), and
the probe: 5′-TCAACCACTGCATTCAGTTGTGATG-3′ (SEQ ID NO: 3);
a 2nd primer and probe set for detecting an adenovirus type C, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-CAGTGGTCTTACATGCAC-3′ (SEQ ID NO: 4),
the downstream primer: 5′-TGGGGTTTCTAAACTTGTTA-3′ (SEQ ID NO: 5), and the probe: 5′-ACGCCTCGGAGTACCTGAGC-3′ (SEQ ID NO: 6);
a 3rd primer and probe set for detecting a varicella-zoster virus (VZV), wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-GGTGTCTCCCTAATCTTG-3′ (SEQ ID NO: 7),
the downstream primer: 5′-CTCTCTTTCTGTGTGTCC-3′ (SEQ ID NO: 8), and
the probe: 5′-AGGCTTCTGCTCTCGACTGG-3′ (SEQ ID NO: 9);
a 4th primer and probe set for detecting an Epstein-Barr virus (EB virus), wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-CTCCCTTTACAACCTCAG-3′ (SEQ ID NO: 10),
the downstream primer: 5′-GACGACTCAATGGTGTAA-3′ (SEQ ID NO: 11), and
the probe: 5′-CCTTGCTGTTCCACAATGTCGT-3′ (SEQ ID NO: 12);
a 5th primer and probe set for detecting a human herpes simplex virus type 1, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-GGATGGCGAACATCTTAA-3′ (SEQ ID NO: 13),
the downstream primer: 5′-GTTGGGTTTGTCCTTCTC-3′ (SEQ ID NO: 14), and
the probe: 5′-ATGGGACCCGTGACACCCTA-3′ (SEQ ID NO: 15);
a 6th primer and probe set for detecting a cytomegalovirus (CMV), wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-CGTGACGTGCATAAAGAG-3′ (SEQ ID NO: 16),
the downstream primer: 5′-GGTGATAGGTGACCAGTA-3′ (SEQ ID NO: 17), and
the probe: 5′-CTCGCAGAAGGACTCCAGGT-3′ (SEQ ID NO: 18);
a 7th primer and probe set for detecting a human herpes virus type 6, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-GTGTGGTAATGGACTAAG-3′ (SEQ ID NO: 19),
the downstream primer: 5′-CGACCATCAAAATATAAAGAG-3′ (SEQ ID NO: 20), and
the probe: 5′-AGCTGCTACAATACACACGTAAGTAC-3′ (SEQ ID NO: 21);
an 8th primer and probe set for detecting Staphylococcus aureus, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-GCGAATGAGCGTTTATTTAG-3′ (SEQ ID NO: 22),
the downstream primer: 5′-GACCTTTAGATAAAGCTGTAATG-3′ (SEQ ID NO: 23), and
the probe: 5′-CGCCATAACTCATACCAGATTGTCCTA-3′ (SEQ ID NO: 24);
a 9th primer and probe set for detecting Streptococcus pneumoniae, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-CAGCTACCAACGACAGTC-3′ (SEQ ID NO: 25),
the downstream primer: 5′-CGCAAGAAGAGTGGGATTA-3′ (SEQ ID NO: 26), and
the probe: 5′-TCTCTAACAAGGTCTCATCCACTACGA-3′ (SEQ ID NO: 27);
a 10th primer and probe set for detecting Staphylococcus epidermidis, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-CGGTGCTTATACTGGAGA-3′ (SEQ ID NO: 28),
the downstream primer: 5′-TCGTCAGTTTCGTGGAAA-3′ (SEQ ID NO: 29), and
the probe: 5′-ACGACGCTCTGAGTGACCAAT-3′ (SEQ ID NO: 30);
an 11th primer and probe set for detecting Pseudomonas aeruginosa, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-TCACCACCTTCTACTTCG-3′ (SEQ ID NO: 31),
the downstream primer: 5′-CAGAGCCATGTTGTACTC-3′ (SEQ ID NO: 32), and
the probe: 5′-ACGACAGCTCCGACCTGAAG-3′ (SEQ ID NO: 33);
a 12th primer and probe set for detecting Acinetobacter baumannii, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-TCCAGTTTATCAAGATTTAGC-3′ (SEQ ID NO: 34),
the downstream primer: 5′-CTTGGGTACCGATATCTG-3′ (SEQ ID NO: 35), and
the probe: 5′-CATAACCAACACGCTTCACTTCCTTAG-3′ (SEQ ID NO: 36);
a 13th primer and probe set for detecting Escherichia coli, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-CCGCAAGAAAACTATCCC-3′ (SEQ ID NO: 37),
the downstream primer: 5′-TAGCGACTGATATTGAACTG-3′ (SEQ ID NO: 38), and
the probe: 5′-CCGCCTTACTGCCGCCTGTT-3′ (SEQ ID NO: 39);
a 14th primer and probe set for detecting Enterococcus faecalis, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-TGCCATCATTTAGTTGGG-3′ (SEQ ID NO: 40),
the downstream primer: 5′-GAAGCTTTTAAGAGATTTGCATG-3′ (SEQ ID NO: 41), and
the probe: 5′-CGGTCTAGCGACTCGTTGTACTTC-3′ (SEQ ID NO: 42);
a 15th primer and probe set for detecting a Mycobacterium tuberculosis complex, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-GTCGAGTACGCCTTCTTG-3′ (SEQ ID NO: 43),
the downstream primer: 5′-CACCTATGTGTCGACCTG-3′ (SEQ ID NO: 44), and
the probe: 5′-CCTTTGTCACCGACGCCTACG-3′ (SEQ ID NO: 45);
a 16th primer and probe set for detecting Enterococcus faecium, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-CATCCAAGATATTCTGCC-3′ (SEQ ID NO: 46),
the downstream primer: 5′-CGGATTTTGTTCAATACAAG-3′ (SEQ ID NO: 47), and
the probe: 5′-CACCATCTACATCGTCAGCAATAATCA-3′ (SEQ ID NO: 48);
a 17th primer and probe set for detecting Clostridium difficile, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-GCGTAGATATTAGGAGGAA-3′ (SEQ ID NO: 49),
the downstream primer: 5′-GGAGTACTTAATGCGTTAG-3′ (SEQ ID NO: 50), and
the probe: 5′-TCTCTGGACTGTAACTGACGCTG-3′ (SEQ ID NO: 51);
an 18th primer and probe set for detecting Stenotrophomonas maltophilia, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-TCCTCCAAGGCTAAATAC-3′ (SEQ ID NO: 52),
the downstream primer: 5′-CGGTTTCAGGTTCTATTTC-3′ (SEQ ID NO: 53), and
the probe: 5′-TGACCGACCGATAGTGAACCAG-3′ (SEQ ID NO: 54);
a 19th primer and probe set for detecting Haemophilus influenzae, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-CGTTCAAATCTTAGACGC-3′ (SEQ ID NO: 55),
the downstream primer: 5′-CGATGTTGTATTCTGGTG-3′ (SEQ ID NO: 56), and
the probe: 5′-CGTTCATCAGTGTTACCTTCTACTAAT-3′ (SEQ ID NO: 57);
a 20th primer and probe set for detecting Klebsiella pneumoniae, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-CCCGTGAACCTTTACTATA-3′ (SEQ ID NO: 58),
the downstream primer: 5′-AAGGTGGTATTTCAAGG-3′ (SEQ ID NO: 59), and
the probe: 5′-TGACACTGAACATTGAGCCTTGAT-3′ (SEQ ID NO: 60);
a 21st primer and probe set for detecting Cryptococcus neoformans, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-CTGGAAGCTCATTTACCTA-3′ (SEQ ID NO: 61),
the downstream primer: 5′-GGCCATGGAATTGGAATA-3′ (SEQ ID NO: 62), and
the probe: 5′-ATTCACCGTAAGCCATCAATCAGG-3′ (SEQ ID NO: 63);
a 22nd primer and probe set for detecting Candida albicans, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-GAGGTCTAAACTTACAACC-3′ (SEQ ID NO: 64),
the downstream primer: 5′-CGTTGTTGAAAGTTTTGAC-3′ (SEQ ID NO: 65), and
the probe: 5′-TCAACTTGTCACACCAGATTATTACTT-3′ (SEQ ID NO: 66); and
a 23rd primer and probe set for detecting Aspergillus fumigatus, wherein sequences of an upstream primer, a downstream primer, and a probe are as follows:
the upstream primer: 5′-TCCCTCTTATCCATTTTCC-3′ (SEQ ID NO: 67),
the downstream primer: 5′-GTTCCAAAGCCGAAAGAATA-3′ (SEQ ID NO: 68), and
the probe: 5′-CCTCATCACTGCAACTCTAATCCTCG-3′ (SEQ ID NO: 69).
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