	US 12,448,656 B2
	*Methods and compositions to select and/or predict cotton plants resistant to Fusarium* race-4 resistance in cotton**
Ramesh Buyyarapu, Waukee, IA (US); Kelly Parliament, Ankeny, IA (US); Joel F Mahill, Bakersfield, CA (US); and Timothy J. Anderson, Bakersfield, CA (US)
Assigned to CORTEVA AGRISCIENCE LLC, Indianapolis, IN (US)
Appl. No. 17/417,431
Filed by CORTEVA AGRISCIENCE LLC, Indianapolis, IN (US)
PCT Filed Dec. 20, 2019, PCT No. PCT/US2019/067825 § 371(c)(1), (2) Date Jun. 23, 2021, PCT Pub. No. WO2020/139756, PCT Pub. Date Jul. 2, 2020.
Claims priority of provisional application 62/785,300, filed on Dec. 27, 2018.
Prior Publication US 2022/0195537 A1, Jun. 23, 2022
Int. Cl. C12Q 1/6895 (2018.01); C12N 15/10 (2006.01); C40B 40/06 (2006.01)

CPC C12Q 1/6895 (2013.01) [C12N 15/1034 (2013.01); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01)]

2 Claims

1. A method for producing a Gossypium plant comprising Fusarium wilt Race 4 (FOV-R4) resistance, the method comprising:

a. extracting a nucleic acid sample from each of one or more Gossypium plants or germplasm thereof;

b. screening each nucleic acid sample for a Fusarium wilt Race 4 (FOV-R4) resistance marker allele C at position 199 of SEQ ID NO: 83;

C. selecting a first Gossypium plant or germplasm thereof identified as having the Fusarium wilt Race 4 (FOV-R4) resistance marker allele screened for in step b; and

d. crossing the first plant selected in step c. with a second Gossypium plant to produce progeny plants, wherein at least one of the progeny plants comprises the Fusarium wilt Race 4 (FOV-R4) resistance marker allele.