US 12,448,655 B2
Antibiotic susceptibility of microorganisms and related methods and systems
Rustem F. Ismagilov, Altadena, CA (US); Nathan G. Schoepp, Burlingame, CA (US); Emily S. Savela, Pasadena, CA (US); Eric J. Liaw, Pasadena, CA (US); Alexander Winnett, Pasadena, CA (US); and Matthew M. Cooper, Pasadena, CA (US)
Assigned to CALIFORNIA INSTITUTE OF TECHNOLOGY, Pasadena, CA (US)
Filed by CALIFORNIA INSTITUTE OF TECHNOLOGY, Pasadena, CA (US)
Filed on Feb. 1, 2021, as Appl. No. 17/164,674.
Application 17/164,674 is a continuation in part of application No. 16/158,233, filed on Oct. 11, 2018, granted, now 11,827,944.
Claims priority of provisional application 62/968,735, filed on Jan. 31, 2020.
Claims priority of provisional application 62/722,124, filed on Aug. 23, 2018.
Claims priority of provisional application 62/571,128, filed on Oct. 11, 2017.
Prior Publication US 2021/0301326 A1, Sep. 30, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/689 (2018.01)
CPC C12Q 1/689 (2013.01) 31 Claims
 
1. A method to detect a nucleic acid of a microorganism in a plurality of samples of an isolate or specimen including the microorganism, the method comprising
contacting the plurality of samples with an antibiotic to provide a plurality of antibiotic-treated samples, and
quantitatively detecting in the plurality of antibiotic-treated samples an accessible nucleic acid of the microorganism, the quantitatively detecting independently performed
in the absence of a lysis treatment targeting the microorganism in an antibiotic-treated sample of the plurality of antibiotic-treated samples, and/or
in the presence of a lysis treatment in an antibiotic-treated sample of the plurality of antibiotic-treated samples, following pre-lysis mechanical separation of the microorganism from the nucleic acid in the antibiotic-treated sample of the plurality of antibiotic-treated samples subjected to lysis treatment to remove the inaccessible nucleic acids from the sample and
the contacting and quantitatively detecting performed to obtain a detected accessible antibiotic-treated nucleic acid concentration value of the microorganism for each antibiotic-treated sample of the plurality of antibiotic-treated samples.