US 12,448,652 B2
Methods and compositions for synthetic biomarkers
David Suhy, San Ramon, CA (US); Paul Escarpe, San Ramon, CA (US); Cyriac Roeding, Portola Valley, CA (US); Justin Lin, Simi Valley, CA (US); Alex Harwig, South San Francisco, CA (US); Shireen Rudina, South San Francisco, CA (US); and Leland Harrison Hartwell, Seattle, WA (US)
Assigned to EARLI Inc., South San Francisco, CA (US)
Filed by EARLI Inc., South San Francisco, CA (US)
Filed on Aug. 18, 2023, as Appl. No. 18/452,504.
Application 18/452,504 is a continuation of application No. 17/219,666, filed on Mar. 31, 2021, granted, now 12,060,613.
Application 17/219,666 is a continuation of application No. PCT/US2020/026758, filed on Apr. 4, 2020.
Claims priority of provisional application 62/955,925, filed on Dec. 31, 2019.
Claims priority of provisional application 62/830,279, filed on Apr. 5, 2019.
Prior Publication US 2025/0060367 A1, Feb. 20, 2025
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6883 (2018.01); A61K 48/00 (2006.01); A61P 35/04 (2006.01); C12N 15/85 (2006.01); C12Q 1/28 (2006.01); C12Q 1/42 (2006.01); C12Q 1/66 (2006.01); C12Q 1/6886 (2018.01); G01N 33/574 (2006.01)
CPC C12Q 1/6883 (2013.01) [A61K 48/0025 (2013.01); A61P 35/04 (2018.01); C12N 15/85 (2013.01); C12Q 1/28 (2013.01); C12Q 1/42 (2013.01); C12Q 1/66 (2013.01); C12Q 1/6886 (2013.01); G01N 33/57484 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/178 (2013.01)] 17 Claims
 
1. A method of expressing a reporter protein in a human subject, comprising:
(a) intravenously administering a plurality of DNA molecules to a human subject suspected of having a cancer in a lung tissue, wherein said plurality of DNA molecules comprises a nanoplasmid or a linear double-stranded DNA molecule that comprises a functional cancer-inducible promoter comprising a family with sequence similarity 111 member B (FAM111B) promoter or a fragment thereof, driving expression of a reporter gene operably linked to said functional cancer-inducible promoter in a lung tissue cancer cell of said human subject, wherein said plurality of DNA molecules is intravenously administered in an amount sufficient to induce expression of a reporter protein encoded by said reporter gene in said lung tissue cancer cell of said human subject; and
(b) expressing said reporter protein in said lung tissue cancer cell of said human subject.