	US 12,448,639 B2
	Methods for producing heme peroxidases
Diana Humer, Vienna (AT); Oliver Spadiut, Vienna (AT); and Julian Ebner, Vienna (AT)
Assigned to TECHNISCHE UNIVERSITÄT WIEN, Vienna (AT)
Appl. No. 17/914,054
Filed by TECHNISCHE UNIVERSITÄT WIEN, Vienna (AT)
PCT Filed Mar. 24, 2021, PCT No. PCT/EP2021/057532 § 371(c)(1), (2) Date Sep. 23, 2022, PCT Pub. No. WO2021/191253, PCT Pub. Date Sep. 30, 2021.
Claims priority of application No. 20165131 (EP), filed on Mar. 24, 2020; application No. 20167716 (EP), filed on Apr. 2, 2020; and application No. 20177549 (EP), filed on May 29, 2020.
Prior Publication US 2023/0122727 A1, Apr. 20, 2023
Int. Cl. C12N 9/08 (2006.01); C12N 1/20 (2006.01); C12N 11/02 (2006.01); C12P 21/02 (2006.01)

CPC C12P 21/02 (2013.01) [C12N 1/205 (2021.05); C12N 9/0065 (2013.01); C12N 11/02 (2013.01); C12Y 111/01007 (2013.01)]

20 Claims

1. Method for producing a heme peroxidase from inclusion bodies (IBs) comprising the steps of:

providing the heme peroxidase in the form of IBs;

solubilizing said IBs;

transferring said solubilized IBs into a refolding buffer to obtain a refolding mix;

adding a heme cofactor to said refolding mix,

wherein the addition of the heme cofactor to the refolding mix is distributed over a time period of at least 1 hour.