US 12,448,637 B2
Primer, device for producing double-stranded DNA using primer, and method for producing double-stranded DNA using primer
Hiroshi Abe, Nagoya (JP); Naoko Abe, Nagoya (JP); Kosuke Nakamoto, Nagoya (JP); Hiroki Murase, Nagoya (JP); and Yasuaki Kimura, Nagoya (JP)
Assigned to Japan Science and Technology Agency, Kawaguchi (JP)
Appl. No. 17/631,407
Filed by Japan Science and Technology Agency, Kawaguchi (JP)
PCT Filed Jul. 31, 2020, PCT No. PCT/JP2020/029442
§ 371(c)(1), (2) Date Jan. 28, 2022,
PCT Pub. No. WO2021/020562, PCT Pub. Date Feb. 4, 2021.
Claims priority of application No. 2019-140852 (JP), filed on Jul. 31, 2019.
Prior Publication US 2022/0282294 A1, Sep. 8, 2022
Int. Cl. C12P 19/34 (2006.01); C07H 19/10 (2006.01); C07H 19/20 (2006.01)
CPC C12P 19/34 (2013.01) [C07H 19/10 (2013.01); C07H 19/20 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A primer for amplifying a nucleic acid, having a structure represented by the following formula (1):

OG Complex Work Unit Chemistry
where B represents a base, R1 represents a decomposable protecting group, R2 represents hydrogen or a hydroxyl group, and a symbol * represents a bond to a sugar of an adjacent nucleotide,
wherein the R1 is a photodecomposable protecting group represented by the following formula (2A) or a reducing agent-decomposable protecting group represented by the following formula (2B):

OG Complex Work Unit Chemistry

OG Complex Work Unit Chemistry
where A1 represents an alkylene group having 1 to 3 carbon atoms and may have a branched chain having 1 to 20 carbon atoms, and a symbol * of formula (2A) represents a bond to oxygen (O) of phosphoric acid,
where A2 represents an alkylene group having 1 to 3 carbon atoms and may have a branched chain having 1 to 20 carbon atoms, and a symbol * of formula (2B) represents a bond to oxygen (O) of phosphoric acid.