US 12,448,634 B2
Method for preparing V-type granular porous starch
Xing Zhou, Wuxi (CN); Zhengyu Jin, Wuxi (CN); Qing Chang, Wuxi (CN); Ren Wang, Wuxi (CN); Jinpeng Wang, Wuxi (CN); Jianwei Zhao, Wuxi (CN); Aiquan Jiao, Wuxi (CN); and Jie Long, Wuxi (CN)
Assigned to JIANGNAN UNIVERSITY, Wuxi (CN)
Filed by Jiangnan University, Wuxi (CN)
Filed on Dec. 31, 2021, as Appl. No. 17/566,724.
Claims priority of application No. 202011632449.1 (CN), filed on Dec. 31, 2020.
Prior Publication US 2022/0243238 A1, Aug. 4, 2022
Int. Cl. C12P 19/04 (2006.01); C08B 30/12 (2006.01); C12P 19/14 (2006.01)
CPC C12P 19/04 (2013.01) [C08B 30/12 (2013.01); C12P 19/14 (2013.01)] 10 Claims
 
1. A method, comprising:
mixing starch and a first ethanol aqueous solution at a temperature of 90-150° C. to yield a mixture of a V-type granular starch and the first ethanol aqueous solution; and
adding a mixed enzyme comprising alpha-amylase and amyloglucosidase to the mixture of the V-type granular starch and the first ethanol aqueous solution, to enzymatically hydrolyze the V-type granular starch to yield a starch dispersion, wherein an addition amount of the mixed enzyme is 30,000-200,000 U per gram of the V-type granular starch; and vacuum-filtering the starch dispersion, washing for 2 to 3 times with a second ethanol aqueous solution with an ethanol concentration of 95% v/v, drying at 110° C. for 2 h, grinding, and sieving to yield V-type granular porous starch having a specific surface area of 9.99-39.94 m2/g and an average pore size of 26.61-27.61 nm.