	US 12,448,633 B2
	Bacterial N-demthylases as biocatalysts for the production of methylxanthines
Ryan M. Summers, Northport, AL (US); Shelby Mills, Tuscaloosa, AL (US); and Meredith Mock, Tuscaloosa, AL (US)
Assigned to The Board of Trustees of The University of Alabama, Tuscaloosa, AL (US)
Filed by The Board of Trustees of The University of Alabama, Tuscaloosa, AL (US)
Filed on Aug. 8, 2022, as Appl. No. 17/883,115.
Claims priority of provisional application 63/230,466, filed on Aug. 6, 2021.
Prior Publication US 2023/0193332 A1, Jun. 22, 2023
Int. Cl. C12P 17/14 (2006.01); C12N 1/21 (2006.01); C12N 9/02 (2006.01)

CPC C12P 17/14 (2013.01) [C12N 9/0071 (2013.01); C12Y 114/00 (2013.01)]

5 Claims

1. A method of preparing paraxanthine (1,7-dimethylxanthine) and/or 7-methylxanthine, comprising: contacting a bacterial strain expressing N-demethylase or a mutant thereof with caffeine, theobromine, or theophylline; wherein the bacterial strain also overexpresses frmAB.