US 12,448,626 B2
Compositions and methods for increased protein production in Bacillus licheniformis
Marc Anton Bernhard Kolkman, Oegstgeest (NL); Steven D. Doig, Wilmington, DE (US); Ryan L. Frisch, Newark, DE (US); Hongxian He, Wilmington, DE (US); Frank Wouter Koopman, Utrecht (NL); and Chris Leeflang, Twisk (NL)
Assigned to DANISCO US INC., Palo Alto, CA (US)
Filed by DANISCO US INC., Palo Alto, CA (US)
Filed on Nov. 30, 2023, as Appl. No. 18/524,856.
Application 18/524,856 is a division of application No. 16/486,892, granted, now 11,866,713, previously published as PCT/US2018/019140, filed on Feb. 22, 2018.
Claims priority of provisional application 62/463,268, filed on Feb. 24, 2017.
Prior Publication US 2024/0182914 A1, Jun. 6, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/75 (2006.01); C07K 14/32 (2006.01); C12P 21/02 (2006.01)
CPC C12N 15/75 (2013.01) [C07K 14/32 (2013.01); C12P 21/02 (2013.01)] 4 Claims
 
1. A modified Bacillus licheniformis cell derived from a parental B. licheniformis cell, wherein the modified cell comprises a genetic modification which disrupts, deletes, inactivates, or down-regulates:
(i) a rghR2 gene encoding a RghR2 protein comprising at least 90% sequence identity to SEQ ID NO: 4,
(ii) a rghR1 gene encoding a RghR1 protein comprising at least 90% sequence identity to SEQ ID NO: 16,
(iii) a Bli03644 gene encoding a Bli03644 protein comprising at least 90% sequence identity to SEO ID NO: 20,
(iv) a rghR2 gene encoding a RghR2 protein comprising at least 90% sequence identity to SEQ ID NO: 4; and a rghR1 gene encoding a RghR1 protein comprising at least 90% sequence identity to SEQ ID NO: 16,
(v) a rghR2 gene encoding a RghR2 protein comprising at least 90% sequence identity to SEQ ID NO: 4, a rghR1 gene encoding a RghR1 protein comprising at least 90% sequence identity to SEQ ID NO: 16, and a yvzC gene encoding a yvzC protein comprising at least 90% sequence identity to SEQ ID NO: 18, or
(vi) a rghR2 gene encoding a RghR2 protein comprising at least 90% sequence identity to SEO ID NO: 4, a rghR1 gene encoding a RghR1 protein comprising at least 90% sequence identity to SEO ID NO: 16, a yvzC gene encoding a yvzC protein comprising at least 90% sequence identity to SEO ID NO: 18 and a Bli03644 gene encoding a Bli03644 protein comprising at least 90% sequence identity to SEO ID NO: 20,
wherein the modified cell produces an increased amount of a protein of interest relative to the parental cell.