	US 12,448,619 B2
	Treating and preventing microbial infections
Morten Sommer, London (GB); Virginia Martinez, Copenhagen Ø (DK); Eric Van Der Helm, London (GB); Jakob Krause Haaber, Copenhagen Ø (DK); Ana De Santiago Torio, Copenhagen Ø (DK); Christian Grøndahl, Copenhagen Ø (DK); and Jasper Clube, London (GB)
Assigned to SNIPR BIOME APS, Copenhagen Ø (DK)
Filed by SNIPR Biome ApS, Copenhagen Ø (DK)
Filed on May 20, 2022, as Appl. No. 17/750,083.
Application 17/750,083 is a continuation of application No. 17/029,860, filed on Sep. 23, 2020, granted, now 11,485,973.
Application 17/029,860 is a continuation of application No. 16/700,856, filed on Dec. 2, 2019, granted, now 10,920,222, issued on Feb. 16, 2021.
Application 16/700,856 is a continuation of application No. 15/967,484, filed on Apr. 30, 2018, granted, now 10,760,075, issued on Sep. 1, 2020.
Prior Publication US 2022/0282245 A1, Sep. 8, 2022
Int. Cl. C12N 15/11 (2006.01); A61K 38/46 (2006.01); A61K 39/395 (2006.01); A61P 31/04 (2006.01); C07K 16/28 (2006.01)

CPC C12N 15/11 (2013.01) [A61K 38/465 (2013.01); A61K 39/3955 (2013.01); A61K 39/39558 (2013.01); A61P 31/04 (2018.01); C07K 16/2818 (2013.01); C07K 16/2827 (2013.01); C12N 2310/20 (2017.05); C12N 2320/30 (2013.01); C12N 2320/31 (2013.01); Y02A 50/30 (2018.01)]

12 Claims

1. A method of reducing growth of a first Propionibacterium bacteria species or strain in a skin microbiota, the method comprising selectively killing the first Propionibacterium bacteria species or strain comprised by the subject by cutting a target site comprised by the genomes of the first Propionibacterium bacteria species or strain,

wherein the method comprises administering to the subject a first nucleic acid sequence comprising a guide RNA or a DNA encoding the guide RNA,

wherein the cutting is carried out using a Cas nuclease that is programmed by the guide RNA to cut the target site,

wherein the subject comprises second bacteria of one or more strains or species that are different from that of the first Propionibacterium bacteria species or strain,

wherein the genomes of the second bacteria do not comprise the target site,

wherein the genomes of the second bacteria are not cut by the Cas nuclease in the subject, whereby second bacteria survive in the presence of the Cas nuclease in the subject,

and wherein the reduction of the growth of the first bacteria is maintained for at least 60 minutes after exposing the subject to the guide RNA.