US 12,448,617 B2
Specific electroporation and lysis of eukaryotic cells
Klemens Wassermann, Korneuburg (AT); Terje Wimberger, Vienna (AT); and Johannes Peham, Vienna (AT)
Assigned to AIT AUSTRIAN INSTITUTE OF TECHNOLOGY GMBH, Vienna (AT)
Appl. No. 17/312,837
Filed by AIT AUSTRIAN INSTITUTE OF TECHNOLOGY GMBH, Vienna (AT)
PCT Filed Dec. 12, 2019, PCT No. PCT/EP2019/084793
§ 371(c)(1), (2) Date Jun. 10, 2021,
PCT Pub. No. WO2020/120651, PCT Pub. Date Jun. 18, 2020.
Claims priority of application No. 18211969 (EP), filed on Dec. 12, 2018.
Prior Publication US 2022/0049239 A1, Feb. 17, 2022
Int. Cl. C12N 13/00 (2006.01); C12M 1/00 (2006.01); C12M 1/42 (2006.01)
CPC C12N 13/00 (2013.01) [C12M 35/02 (2013.01); C12M 47/06 (2013.01)] 17 Claims
 
1. A method of targeted electroporation and/or lysis of eukaryotic cellular bodies in a biological sample with at least two subgroups of eukaryotic cellular bodies, wherein the at least two subgroups are from a mammal and each subgroup has a different susceptibility to electroporation and/or lysis in electric fields, comprising the following steps:
transferring the biological sample in a chamber;
exposing the biological sample to an electric field in the chamber, wherein the electric field is generated by at least two electrodes which are coated with a dielectric material with a relative permittivity greater than 3.9 at least covering the surfaces of the electrodes that face the interior of the chamber; and
lysing and/or electroporating one of the subgroups in the electric field, wherein the electric field has a field strength, frequency or wave form for electroporation and/or lysis of the subgroup and each subgroup of the at least two subgroups is differently affected by said electric field than another of the at least two subgroups.