	US 12,448,612 B2
	Mutant polymerases and methods of using the same
Arvydas Lubys, Vilnius (LT); Zana Kapustina, Vilnius (LT); and Aiste Jaspone, Vilnius (LT)
Assigned to Thermo Fisher Scientific Baltics UAB, Vilnius (LT)
Appl. No. 17/924,637
Filed by Thermo Fisher Scientific Baltics UAB, Vilnius (LT)
PCT Filed May 11, 2021, PCT No. PCT/EP2021/062559 § 371(c)(1), (2) Date Nov. 10, 2022, PCT Pub. No. WO2021/228905, PCT Pub. Date Nov. 18, 2021.
Claims priority of provisional application 63/023,026, filed on May 11, 2020.
Prior Publication US 2023/0174957 A1, Jun. 8, 2023
Int. Cl. C12N 9/12 (2006.01); C12P 19/34 (2006.01)

CPC C12N 9/1247 (2013.01) [C12P 19/34 (2013.01); C12Y 207/07006 (2013.01)]

6 Claims

1. A mutant polymerase comprising:

a catalytic domain having at least 95% sequence identity with SEQ ID NO: 4, and wherein the mutant polymerase comprises one or more mutations at position V459, G231, and/or V365 relative to SEQ ID NO: 4.