US 12,448,607 B2
Systems and methods for multilane vasculature
Dongeun Huh, Villanova, PA (US); and Andrei Georgescu, Philadelphia, PA (US)
Assigned to The Trustees of the University of Pennsylvania, Philadelphia, PA (US)
Appl. No. 17/041,110
Filed by THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA, Philadelphia, PA (US)
PCT Filed Mar. 26, 2019, PCT No. PCT/US2019/024093
§ 371(c)(1), (2) Date Sep. 24, 2020,
PCT Pub. No. WO2019/191111, PCT Pub. Date Oct. 3, 2019.
Claims priority of provisional application 62/648,209, filed on Mar. 26, 2018.
Prior Publication US 2021/0108178 A1, Apr. 15, 2021
Int. Cl. C12N 5/071 (2010.01); A61L 27/36 (2006.01); A61L 27/50 (2006.01); C12M 1/12 (2006.01); C12M 3/00 (2006.01)
CPC C12N 5/0691 (2013.01) [A61L 27/3687 (2013.01); A61L 27/507 (2013.01); C12M 21/08 (2013.01); C12M 25/14 (2013.01); C12N 5/0677 (2013.01); C12N 2500/02 (2013.01); C12N 2501/165 (2013.01); C12N 2513/00 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A method for culturing a tissue using a microphysiological device, comprising:
embedding a hydrogel into a central channel of the microphysiological device, thereby forming a three-dimensional structure within the central channel;
seeding vascular cells within a first channel adjacent the central channel and onto a first wall of the three-dimensional structure formed within the central channel, wherein the vascular cells seeded within the first channel form a first vascular interface between the three-dimensional structure and the first channel that permits perfusion between the first channel and the three-dimensional structure;
seeding vascular cells within a second channel adjacent the central channel and onto a second wall of the three-dimensional structure formed within the central channel, wherein the vascular cells seeded within the second channel form a second vascular interface between the three-dimensional structure and the second channel that permits perfusion between the second channel and the three-dimensional structure; and
perfusing culture medium laterally through the microphysiological device from the first channel to the second channel via the central channel, the central channel being arranged between the first channel and the second channel,
wherein the perfused culture medium is provided via the first channel and is removed via the second channel.