US 12,448,605 B2
Encapsulation and cardiac differentiation of hiPSCs in 3D PEG-fibrinogen hydrogels
Elizabeth A. Lipke, Auburn, AL (US); Petra Kerscher, Auburn, AL (US); and Alexander J. Hodge, Auburn, AL (US)
Assigned to Auburn University, Auburn, AL (US)
Filed by Auburn University, Auburn, AL (US)
Filed on Jun. 24, 2022, as Appl. No. 17/808,873.
Application 17/808,873 is a continuation of application No. 16/420,714, filed on May 23, 2019, granted, now 11,371,021.
Application 16/420,714 is a continuation of application No. 15/411,807, filed on Jan. 20, 2017, granted, now 10,301,597, issued on May 28, 2019.
Application 15/411,807 is a continuation of application No. 14/538,435, filed on Nov. 11, 2014, granted, now 9,587,221, issued on Mar. 7, 2017.
Claims priority of provisional application 61/902,453, filed on Nov. 11, 2013.
Prior Publication US 2022/0403338 A1, Dec. 22, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/077 (2010.01); C12N 5/074 (2010.01)
CPC C12N 5/0657 (2013.01) [C12N 5/0696 (2013.01); C12N 2500/46 (2013.01); C12N 2500/50 (2013.01); C12N 2501/999 (2013.01); C12N 2506/45 (2013.01); C12N 2513/00 (2013.01); C12N 2533/52 (2013.01); C12N 2533/54 (2013.01); C12N 2537/10 (2013.01)] 20 Claims
 
1. A method of screening a candidate compound or substance for an effect on cardiac tissue, the method comprising:
forming a biomimetic-PSC suspension by suspending a population of pluripotent stem cells (PSCs) in a covalently crosslinkable component and a natural hydrogel component, the natural hydrogel component comprising one or more of fibrinogen, collagen, gelatin, hyaluronic acid, elastin, fibronectin, laminin, fibrin, alginate, and decellularized cardiac extracellular matrix;
crosslinking the biomimetic-PSC suspension to produce a three-dimensional biomimetic PSC microenvironment;
culturing the three-dimensional biomimetic-PSC microenvironment to differentiate the three-dimensional biomimetic-PSC microenvironment into a three-dimensional, synchronously contracting cardiac tissue;
exposing the cardiac tissue to a candidate compound or substance; and
determining an effect of the candidate compound or substance on the cardiac tissue.