US 12,448,601 B2
Neural tissue unit and use of such a unit for implantation into the nervous system of a mammal
Maxime Feyeux, Talence (FR); Kevin Alessandri, Bordeaux (FR); Pierre Nassoy, Bordeaux (FR); Laurent Cognet, Bordeaux (FR); Abdelhamid Benazzouz, Pessac (FR); and Erwan Bezard, Bordeaux (FR)
Assigned to UNIVERSITE DE BORDEAUX, Bordeaux (FR); CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); and INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE, Palaiseau (FR)
Appl. No. 16/462,965
Filed by UNIVERSITÉ DE BORDEAUX, Bordeaux (FR); CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); and INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE, Palaiseau (FR)
PCT Filed Nov. 23, 2017, PCT No. PCT/FR2017/053226
§ 371(c)(1), (2) Date May 22, 2019,
PCT Pub. No. WO2018/096278, PCT Pub. Date May 31, 2018.
Claims priority of application No. 1661378 (FR), filed on Nov. 23, 2016.
Prior Publication US 2020/0063099 A1, Feb. 27, 2020
Int. Cl. C12N 5/0793 (2010.01); A61K 35/30 (2015.01); C12N 5/00 (2006.01)
CPC C12N 5/0619 (2013.01) [A61K 35/30 (2013.01); C12N 5/0012 (2013.01); C12N 2501/39 (2013.01); C12N 2501/42 (2013.01); C12N 2506/45 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A method for grafting a neural tissue unit into the nervous system of a human subject in need of treatment for Parkinson's disease, the method comprising:
a) obtaining a neural tissue unit by differentiating a pluripotent stem cell into post-mitotic neuronal cells, wherein the pluripotent stem cell has been seeded within a hydrogel capsule surrounding a microcompartment containing glial cells and extracellular matrix, the pluripotent stem cell being differentiated within the hydrogel capsule, and wherein the hydrogel capsule surrounds the neural tissue unit which comprises glial cells and differentiated post-mitotic neuronal cells immunocompatible with the human subject, said differentiated post-mitotic neuronal cells being organized in a three-dimensional (3D) network embedded in the extracellular matrix;
b) at least partially removing the hydrogel capsule from the neural tissue unit; and
c) implanting the neural tissue unit into the brain of the human subject in need of treatment for Parkinson's disease, wherein subsequent to the implantation, the neural tissue unit projects axons into the human subject's tissue.