US 12,448,407 B2
Medium additive for suspension culture, medium composition and culture method
Takeshi Serizawa, Tokyo (JP); Toshiki Sawada, Tokyo (JP); Masahito Nishiura, Kyoto (JP); and Rina Murase, Kyoto (JP)
Assigned to DKS CO. LTD., Kyoto (JP); and TOKYO INSTITUTE OF TECHNOLOGY, Tokyo (JP)
Appl. No. 17/765,784
Filed by DKS CO. LTD., Kyoto (JP); and TOKYO INSTITUTE OF TECHNOLOGY, Tokyo (JP)
PCT Filed Oct. 23, 2020, PCT No. PCT/JP2020/039831
§ 371(c)(1), (2) Date Mar. 31, 2022,
PCT Pub. No. WO2021/085317, PCT Pub. Date May 6, 2021.
Claims priority of application No. 2019-199228 (JP), filed on Oct. 31, 2019.
Prior Publication US 2022/0389045 A1, Dec. 8, 2022
Int. Cl. C07H 3/06 (2006.01); C08B 15/08 (2006.01); C12N 1/22 (2006.01); C12N 5/00 (2006.01)
CPC C07H 3/06 (2013.01) [C08B 15/08 (2013.01); C12N 1/22 (2013.01); C12N 5/0018 (2013.01); C12N 2533/78 (2013.01)] 13 Claims
 
1. A cell culture method comprising: suspension culturing animal cells in a medium composition comprising a water-insoluble cellulose oligomer, wherein the cellulose oligomer is represented by the following general formula (1):

OG Complex Work Unit Chemistry
wherein A represents an alkyl group having 1 to 12 carbon atom(s), and n represents an average polymerization degree of 5 to 20, wherein said cellulose oligomer is present in an amount sufficient to retain said cells in a suspended state during said suspension culturing.