US 12,448,340 B2
Isobaric stable isotope-containing phosphorylated protein labeling reagent, and preparation method and application thereof
Xiang Gao, Xiamen (CN); Xiaoyu Wang, Xiamen (CN); Chengting Huang, Xiamen (CN); and Yufen Zhao, Xiamen (CN)
Assigned to GUANGDONG PHOBIOLOGY TECH CO., LTD, Shenzhen (CN)
Appl. No. 17/919,496
Filed by GUANGDONG PHOBIOLOGY TECH CO., LTD, Shenzhen (CN)
PCT Filed Apr. 9, 2021, PCT No. PCT/CN2021/086224
§ 371(c)(1), (2) Date Oct. 17, 2022,
PCT Pub. No. WO2021/208818, PCT Pub. Date Oct. 21, 2021.
Claims priority of application No. 202010303845.3 (CN), filed on Apr. 17, 2020.
Prior Publication US 2023/0150897 A1, May 18, 2023
Int. Cl. C07B 59/00 (2006.01); C07K 1/13 (2006.01); G01N 30/06 (2006.01)
CPC C07B 59/008 (2013.01) [C07K 1/13 (2013.01); G01N 30/06 (2013.01); G01N 2440/14 (2013.01); G01N 2458/15 (2013.01)] 14 Claims
OG exemplary drawing
 
1. An isobaric stable isotope-containing phosphorylated protein labeling reagent comprising a structural formula as follows:

OG Complex Work Unit Chemistry
wherein a is 14 or 15; b is 12 or 13; c is 16 or 18; d is 1 or 2; n is 1, 2, 3, 4, or 5; n1 is 0, 1, or 2; R1 is a side chain substituent of an L or D-configuration amino acid with or without a stable isotope; and R2 is a side chain substituent of an amino acid with or without a stable isotope.