US 12,447,514 B2
Optimization of a thermophilic PHB depolymerase for industrial applications
Stephen Quirk, Alpharetta, GA (US)
Assigned to Kimberly-Clark Worldwide, Inc., Neenah, WI (US)
Appl. No. 18/558,849
Filed by Kimberly-Clark Worldwide, Inc., Neenah, WI (US)
PCT Filed May 28, 2021, PCT No. PCT/US2021/034879
§ 371(c)(1), (2) Date Nov. 3, 2023,
PCT Pub. No. WO2022/250695, PCT Pub. Date Dec. 1, 2022.
Prior Publication US 2024/0226974 A1, Jul. 11, 2024
Int. Cl. B09B 3/60 (2022.01); B09B 101/67 (2022.01); B09B 101/75 (2022.01); C12N 9/18 (2006.01)
CPC B09B 3/60 (2022.01) [C12N 9/18 (2013.01); C12Y 301/01075 (2013.01); B09B 2101/67 (2022.01); B09B 2101/75 (2022.01)] 6 Claims
 
1. A method for treatment of a post-consumer product, the method comprising:
contacting a post-consumer product with a polypeptide, the post-consumer product comprising a polyhydroxyalkanoate, the polypeptide catalyzing degradation of the polyhydroxyalkanoate, the contacting taking place at a temperature ef from 45° C. to 90° C.;
wherein the polypeptide comprises a modified polyhydroxyalkanoate depolymerase comprising the amino acid sequence of SEQ ID NO: 6 with the exception of at least one mutation at one or more position corresponding to position 3, 10, 15, 33, 41, 44, 49, 50, 62, 67, 78, 91, 92, 93, 97, 100, 102, 114, 122, 123, 124, 125, 127, 128, 129, 135, 139, 144, 145, 148, 152, 157, 159, 160, 167, 169, 184, 189, 200, 202, 206, 209, 233, 259, 260, 261, 269, and/or 275 of the amino acid sequence of SEQ ID NO: 6, wherein each mutation of the modified polyhydroxyalkanoate depolymerase independently has a DDG value of 1 kcal/mol or greater.