US 12,447,456 B2
Biological sample capture with multiplex analysis
Mikhail Kostylev, New Haven, CT (US); and Erik Gunther, Branford, CT (US)
Assigned to PROTEOWISE INC., New Haven, CT (US)
Filed by ProteoWise Inc., New Haven, CT (US)
Filed on Apr. 29, 2022, as Appl. No. 17/732,932.
Claims priority of provisional application 63/182,345, filed on Apr. 30, 2021.
Prior Publication US 2022/0370976 A1, Nov. 24, 2022
Int. Cl. B01J 19/00 (2006.01); G01N 33/543 (2006.01)
CPC B01J 19/0046 (2013.01) [G01N 33/54306 (2013.01); B01J 2219/00587 (2013.01); B01J 2219/00626 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A method for transferring bio-molecular components of individual cells in a biological sample to a porous substate, the method comprising:
contacting or seeding the biological sample comprising a whole cell or a virus to a first side of the porous substrate having a plurality of interstices or pores extending contiguously from the first side to a second side, wherein the porous substrate underlies the whole cell or virus and is derivatized such that each of the interstices or pores covalently bind and/or couple the bio-molecular components of the whole cell or virus in the biological sample; and
transferring the bio-molecular components of the whole cell or virus in the biological sample to the interstices or pores of the porous substrate, wherein the bio-molecular components of the whole cell or virus covalently affix to the interstices or pores of the porous substrate,
wherein:
the porous substrate is a derivatized porous alumina, a derivatized porous glass, or a derivatized porous polymeric material;
the interstices or pores have a diameter of 500 nm or less; and
the porous substrate has a thickness from the first side to the second side of 50 μm to 100 μm.