	US 12,447,235 B2
	Hydrogel hybrid material, method of its preparation and application
Maria Nowakowska, Cracow (PL); Joanna Lewandowska-Lancucka, Wolbrom (PL); and Adriana Gilarska, Lutkow (PL)
Assigned to UNIWERSYTET JAGIELLONSKI, Cracow (PL)
Appl. No. 17/426,629
Filed by UNIWERSYTET JAGIELLONSKI, Cracow (PL)
PCT Filed Feb. 21, 2020, PCT No. PCT/PL2020/050019 § 371(c)(1), (2) Date Jul. 28, 2021, PCT Pub. No. WO2020/171723, PCT Pub. Date Aug. 27, 2020.
Claims priority of application No. 428993 (PL), filed on Feb. 21, 2019.
Prior Publication US 2022/0047776 A1, Feb. 17, 2022
Int. Cl. A61L 27/44 (2006.01); A61L 27/52 (2006.01); C08L 89/06 (2006.01); C09C 1/30 (2006.01); C09C 3/08 (2006.01)

CPC A61L 27/446 (2013.01) [A61L 27/52 (2013.01); C08L 89/06 (2013.01); C09C 1/3063 (2013.01); C09C 3/08 (2013.01); A61L 2300/802 (2013.01); A61L 2430/02 (2013.01); C08L 2203/02 (2013.01); C08L 2205/03 (2013.01)]

6 Claims

1. A method of preparation of an injectable bioactive hydrogel hybrid material having the ability to initiate an apatite nucleation process comprising the following steps:

a) functionalizing silica particles with amine groups using a sol-gel method by combining tetraethoxysilane and aminopropyltriethoxysilane at a molar ratio of 10:1,

b) dispersing the silica particles functionalized with amine groups obtained in step a) in a mixture of solutions of collagen, chitosan and hyaluronic acid having a weight ratio of 50:40:10 (collagen: chitosan: hyaluronic acid) to prepare a dispersion, and

c) covalently crosslinking the dispersion obtained in step b) with genipin to obtain the injectable bioactive hydrogel hybrid material, wherein the content of the functionalized silica particles in the injectable bioactive hydrogel hybrid material is no less than 1.9 mg/mL.