US 12,447,215 B2
Nanoparticle probes and methods of making and use thereof
Paul C. Lee, Columbia, MD (US)
Filed by Paul C. Lee, Columbia, MD (US)
Filed on Jun. 20, 2023, as Appl. No. 18/211,938.
Application 18/211,938 is a continuation of application No. 17/465,571, filed on Sep. 2, 2021, abandoned.
Application 17/465,571 is a continuation of application No. 16/669,059, filed on Oct. 30, 2019, granted, now 11,135,317, issued on Oct. 5, 2021.
Application 16/669,059 is a continuation of application No. 15/680,016, filed on Aug. 17, 2017, granted, now 10,507,253, issued on Dec. 17, 2019.
Application 15/680,016 is a continuation in part of application No. 15/461,035, filed on Mar. 16, 2017, granted, now 10,507,252, issued on Dec. 17, 2019.
Claims priority of provisional application 62/309,751, filed on Mar. 17, 2016.
Prior Publication US 2024/0100196 A1, Mar. 28, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 49/00 (2006.01); G01N 33/543 (2006.01); G01N 33/58 (2006.01); G01N 33/68 (2006.01)
CPC A61K 49/0093 (2013.01) [G01N 33/54346 (2013.01); G01N 33/587 (2013.01); G01N 33/6842 (2013.01); G01N 33/6893 (2013.01); G01N 2400/40 (2013.01); G01N 2800/042 (2013.01); G01N 2800/321 (2013.01); G01N 2800/347 (2013.01)] 24 Claims
 
1. A nanoparticle probe comprising:
a nanoparticle base structure functionalized with a therapeutic agent, wherein the therapeutic agent is a growth factor; and
a slip bond moiety comprising a ligand for a glycocalyx of a cell;
a polymeric tether functionalized to the nanoparticle base structure and to an associative moiety, wherein said associative moiety comprises a ligand for a cell surface protein, a receptor, or a biomarker; and
wherein the associative moiety preferentially binds to the protein, the receptor, and the biomarker of the cell based on the thickness of a glycocalyx layer of the cell.