US 12,446,573 B2
Tunable, simple, higher-yield, higher-rate, lower-cost recovery method of biopharmaceutical products from cell factories
Naveed Aslam, Bellaire, TX (US)
Assigned to GPS Innovations, Bellaire, TX (US)
Filed by Naveed Aslam, Bellaire, TX (US)
Filed on Feb. 1, 2021, as Appl. No. 16/974,427.
Prior Publication US 2022/0240498 A1, Aug. 4, 2022
Int. Cl. A01N 1/125 (2025.01)
CPC A01N 1/125 (2025.01) 20 Claims
 
1. A continuous process of stimulating the recovery of intercellular products from cell factories comprising:
continuously feeding cell factories into a spray chamber that transports the cell factories to a vessel communicatively coupled to the spray chamber;
spraying the cell factories within the spray chamber with a liquid inert gas in a manner to produce a spray cloud and allow the liquid inert gas to absorb into the cell wall;
pressurizing the vessel with a gaseous inert gas in a manner that allows dissolution of the gaseous inert gas into the cell factories; and
depressurizing the vessel to thereby form intercellular products and cellular debris.