US 12,119,088 B2
Deep neural network-based sequencing
Kishore Jaganathan, San Francisco, CA (US); Anindita Dutta, Brisbane, CA (US); Dorna Kashefhaghighi, Menlo Park, CA (US); John Randall Gobbel, Brisbane, CA (US); and Amirali Kia, San Mateo, CA (US)
Assigned to Illumina, Inc., San Diego, CA (US)
Filed by ILLUMINA, INC., San Diego, CA (US)
Filed on Aug. 30, 2022, as Appl. No. 17/899,539.
Application 17/899,539 is a continuation of application No. 16/826,168, filed on Mar. 21, 2020, granted, now 11,436,429.
Claims priority of provisional application 62/821,724, filed on Mar. 21, 2019.
Claims priority of provisional application 62/821,766, filed on Mar. 21, 2019.
Claims priority of provisional application 62/821,681, filed on Mar. 21, 2019.
Claims priority of provisional application 62/821,602, filed on Mar. 21, 2019.
Claims priority of provisional application 62/821,618, filed on Mar. 21, 2019.
Prior Publication US 2023/0004749 A1, Jan. 5, 2023
Int. Cl. G06F 16/907 (2019.01); G06F 18/21 (2023.01); G06F 18/214 (2023.01); G06F 18/23 (2023.01); G06F 18/23211 (2023.01); G06F 18/24 (2023.01); G06F 18/2415 (2023.01); G06F 18/2431 (2023.01); G06N 3/04 (2023.01); G06N 3/08 (2023.01); G06N 3/084 (2023.01); G06N 7/01 (2023.01); G06V 10/26 (2022.01); G06V 10/44 (2022.01); G06V 10/75 (2022.01); G06V 10/762 (2022.01); G06V 10/764 (2022.01); G06V 10/77 (2022.01); G06V 10/778 (2022.01); G06V 10/82 (2022.01); G06V 10/98 (2022.01); G06V 20/69 (2022.01); G16B 40/00 (2019.01); G16B 40/20 (2019.01); G06N 5/046 (2023.01); G06V 20/40 (2022.01)
CPC G16B 40/20 (2019.02) [G06F 16/907 (2019.01); G06F 18/214 (2023.01); G06F 18/217 (2023.01); G06F 18/23 (2023.01); G06F 18/23211 (2023.01); G06F 18/24 (2023.01); G06F 18/2415 (2023.01); G06F 18/2431 (2023.01); G06N 3/04 (2013.01); G06N 3/08 (2013.01); G06N 3/084 (2013.01); G06N 7/01 (2023.01); G06V 10/267 (2022.01); G06V 10/454 (2022.01); G06V 10/751 (2022.01); G06V 10/763 (2022.01); G06V 10/764 (2022.01); G06V 10/7715 (2022.01); G06V 10/7784 (2022.01); G06V 10/82 (2022.01); G06V 10/993 (2022.01); G06V 20/69 (2022.01); G16B 40/00 (2019.02); G06N 5/046 (2013.01); G06V 20/47 (2022.01)] 20 Claims
OG exemplary drawing
 
1. A sequencing system, comprising:
a receptacle coupled to a biosensor system, the biosensor system configured to comprise an array of light detectors, the biosensor system comprising a biosensor, and the biosensor comprising reaction sites configured to contain analytes;
an illumination system configured to direct excitation light toward the biosensor and illuminate the analytes in the reaction sites, wherein at least some of the analytes provide emission signals when illuminated; and
a system controller coupled to the receptacle and comprising an analysis module, the analysis module configured to:
obtain image data from the light detectors at each of a plurality of sequencing cycles of a sequencing run, wherein the image data is derived from the emission signals detected by the light detectors and comprises images of clusters and their surrounding background,
process the image data for each of the plurality of sequencing cycles through a neural network, and
produce a base call for one or more of the clusters at each of the plurality of sequencing cycles.