US 12,117,455 B2
Early detection marker for degenerative osteoarthritis with TRIM24-RIP3 axis
You-Sun Kim, Suwon-si (KR); and Siyoung Yang, Suwon-si (KR)
Assigned to AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Suwon-si (KR)
Filed by AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Suwon-si (KR)
Filed on Sep. 15, 2021, as Appl. No. 17/475,753.
Claims priority of application No. 10-2020-0119158 (KR), filed on Sep. 16, 2020.
Prior Publication US 2022/0091136 A1, Mar. 24, 2022
Int. Cl. C12Q 1/6883 (2018.01); G01N 33/68 (2006.01)
CPC G01N 33/6893 (2013.01) [G01N 2500/00 (2013.01); G01N 2800/105 (2013.01)] 5 Claims
 
1. A method for diagnosing osteoarthritis of a test subject, comprising:
(a) obtaining a biosample from the test subject;
(b) contacting the biosample of the test subject with an agent for measuring an expression or activity level of TRIM24 and an agent for measuring an expression or activity level of RIP3;
(c) determining that the expression or activity level of TRIM24 of the biosample of the test subject is lower than that of a normal biosample and that the expression or activity level of RIP3 of the biosample of the test subject is higher than that of a normal biosample; and
(d) diagnosing the test subject as having osteoarthritis,
wherein the agent for measuring an expression or activity level of the RIP3 is a primer set comprising a first primer comprising the nucleotide sequence of SEQ ID NO: 17 and a second primer comprising the nucleotide sequence of SEQ ID NO: 18, and
wherein the agent for measuring an expression or activity level of the TRIM24 is a primer set comprising a first primer comprising the nucleotide sequence of SEQ ID NO: 23 and a second primer comprising the nucleotide sequence of SEQ ID NO: 24.