US 12,117,440 B2
Method for isolating exosome and exosome isolation kit
Akio Kuroda, Higashi-Hiroshima (JP); and Takenori Ishida, Higashi-Hiroshima (JP)
Assigned to Hiroshima University, Higashi-Hiroshima (JP)
Appl. No. 16/640,753
Filed by Hiroshima University, Higashi-Hiroshima (JP)
PCT Filed Jul. 25, 2018, PCT No. PCT/JP2018/027945
§ 371(c)(1), (2) Date Jun. 8, 2021,
PCT Pub. No. WO2019/039179, PCT Pub. Date Feb. 28, 2019.
Claims priority of application No. 2017-159645 (JP), filed on Aug. 22, 2017.
Prior Publication US 2021/0389314 A1, Dec. 16, 2021
Int. Cl. G01N 33/543 (2006.01); C07K 7/06 (2006.01)
CPC G01N 33/54326 (2013.01) [C07K 7/06 (2013.01)] 5 Claims
 
1. A method for isolating an exosome from a sample containing the exosome, said method comprising:
a complex forming step of forming a complex by binding the exosome to a peptide supported by a carrier, the peptide containing four or more lysines which are close to each other, the carrier being capable of supporting the peptide and, in binding the exosome to the peptide, (i) the sample, the peptide, and the carrier being brought into contact with each other or (ii) the sample and the peptide supported by the carrier being brought into contact with each other; and
a dissociating step of dissociating the exosome from the complex by bringing the complex which has been obtained in the complex forming step into contact with a dissociation buffer containing metal cations.