US 12,117,438 B2
Multivalent binding composition for nucleic acid analysis
Sinan Arslan, San Diego, CA (US); Molly He, San Diego, CA (US); Matthew Kellinger, San Diego, CA (US); Jake Levieux, San Diego, CA (US); Michael Previte, San Diego, CA (US); Junhua Zhao, San Diego, CA (US); and Su Zhang, Rosemead, CA (US)
Filed by Element Biosciences, Inc., San Diego, CA (US)
Filed on Jul. 22, 2020, as Appl. No. 16/936,121.
Application 16/936,121 is a continuation of application No. 16/579,794, filed on Sep. 23, 2019, granted, now 10,768,173.
Claims priority of provisional application 62/897,172, filed on Sep. 6, 2019.
Prior Publication US 2021/0072234 A1, Mar. 11, 2021
Int. Cl. G01N 33/53 (2006.01); C12Q 1/6874 (2018.01); G01N 33/58 (2006.01)
CPC G01N 33/5308 (2013.01) [C12Q 1/6874 (2013.01); G01N 33/582 (2013.01)] 20 Claims
 
1. A system comprising:
(a) a flow cell with an interior surface comprising a plurality of primed target nucleic acid sequences coupled thereto, wherein a primed target nucleic acid sequence of said plurality of primed target nucleic acid sequences has a polymerase bound thereto:
(b) a fluid flow controller configured to control sequential and iterative delivery of a reagent to said interior surface of said flow cell;
(c) an imaging module configured to image said interior surface of said flow cell; and
(d) a processor, wherein said processor is programed to instruct said system to perform an iterative method comprising:
(i) contacting said plurality of primed target nucleic acid sequences coupled to said interior surface of said flow cell with a conjugated nucleotide composition to form a binding complex between said plurality of primed target nucleic acid sequences and a plurality of nucleotide moieties of said conjugated nucleotide composition, wherein said binding complex has a persistence time of at least 2 seconds when a nucleotide moiety of said plurality of nucleotide moieties is complementary to a nucleotide of said primed target nucleic acid sequence; and
(ii) imaging said interior surface of said flow cell to detect said binding complex and thereby determine an identity of said nucleotide of said primed target nucleic acid sequence.