US 12,117,426 B2
Biological membrane phosphoinositide separation method
Keiko Matsumoto, Kyoto (JP); Yuta Shimanaka, Tokyo (JP); Nozomu Kono, Tokyo (JP); and Hiroyuki Arai, Tokyo (JP)
Assigned to SHIMADZU CORPORATION, Kyoto (JP); and THE UNIVERSITY OF TOKYO, Tokyo (JP)
Appl. No. 17/272,973
Filed by SHIMADZU CORPORATION, Kyoto (JP); and THE UNIVERSITY OF TOKYO, Tokyo (JP)
PCT Filed Aug. 30, 2019, PCT No. PCT/JP2019/034134
§ 371(c)(1), (2) Date Mar. 3, 2021,
PCT Pub. No. WO2020/054462, PCT Pub. Date Mar. 19, 2020.
Claims priority of application No. 2018-168584 (JP), filed on Sep. 10, 2018.
Prior Publication US 2021/0310999 A1, Oct. 7, 2021
Int. Cl. G01N 30/34 (2006.01); B01D 15/40 (2006.01); B01J 20/24 (2006.01); B01J 20/32 (2006.01); G01N 30/06 (2006.01); G01N 30/16 (2006.01); G01N 30/72 (2006.01)
CPC G01N 30/34 (2013.01) [B01D 15/40 (2013.01); B01J 20/24 (2013.01); B01J 20/3204 (2013.01); G01N 30/06 (2013.01); G01N 30/16 (2013.01); G01N 30/72 (2013.01)] 3 Claims
OG exemplary drawing
 
1. A method of separating non-deacylated phosphatidylinositol 3-phosphate PI(3)P, non-deacylated phosphatidylinositol 4-phosphate PI(4)P, and non-deacylated PI(5)P phosphatidylinositol 5-phosphate from one another, or of separating non-deacylated phosphatidylinositol 3,4-bisphosphate PI(3,4)P2, non-deacylated phosphatidylinositol 3,5-bisphosphate PI(3,5)P2, and non-deacylated phosphatidylinositol 4-5-bisphosphate PI(4,5)P2 from one another using a supercritical fluid chromatograph having an analysis flow path, a silica stationary phase, a separation medium and a separation column packed with the separation medium in which β-cyclodextrin is bound to the silica stationary phase, the method comprising injecting a first sample containing non-deacylated phosphatidylinositol 3-phosphate PI(3)P, non-deacylated phosphatidylinositol 4-phosphate PI(4)P, and non-deacylated phosphatidylinositol 5-phosphate PI(5)P, or injecting a second sample containing non-deacylated phosphatidylinositol 3,4-bisphosphate PI(3,4)P2, non-deacylated PI(3,5)P2, and non-deacylated PI(4,5)P2 into the analysis flow path and thereby separating the non-deacylated phosphatidylinositol 3-phosphate PI(3)P, the non-deacylated phosphatidylinositol 4-phosphate PI(4)P, and the non-deacylated PI(5)P phosphatidylinositol 5-phosphate from one another, or separating the non-deacylated phosphatidylinositol 3,4-bisphosphate PI(3,4)P2, the non-deacylated phosphatidylinositol 3,5-bisphosphate PI(3,5)P2, and the non-deacylated phosphatidylinositol 4-5-bisphosphate PI(4,5)P2 from one another.