US 12,117,399 B2
Method for improved Raman analysis with a monolayer-coated substrate
Mohammad Kamal Hossain, Dhahran (SA)
Assigned to KING FAHD UNIVERSITY OF PETROLEUM AND MINERALS, Dhahran (SA)
Filed by KING FAHD UNIVERSITY OF PETROLEUM AND MINERALS, Dhahran (SA)
Filed on May 17, 2023, as Appl. No. 18/319,055.
Application 18/319,055 is a continuation of application No. 17/828,632, filed on May 31, 2022, granted, now 11,754,502.
Prior Publication US 2023/0384229 A1, Nov. 30, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 21/65 (2006.01); B05D 5/00 (2006.01); B05D 7/24 (2006.01)
CPC G01N 21/658 (2013.01) [B05D 5/00 (2013.01); B05D 7/24 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method for forming a substrate for improved surface-enhanced Raman scattering (SERS) measurement, comprising:
forming a monolayer assembly on a first substrate by:
dropping a 200-400 μL aliquot of a colloid of gold nanoparticles onto the first substrate to form a film;
placing a second substrate onto the film, wherein the colloid is disposed between the first and second substrates and the first and second substrates are spaced 0.1-2 mm apart;
irradiating the colloid disposed between the first and second substrates with infrared light to evaporate the solvent to form the monolayer assembly of gold nanoparticles, wherein the monolayer assembly of gold nanoparticles includes a plurality of strips of gold nanoparticles spaced 25 μm apart and wherein each gold nanoparticle of the plurality of strips of gold nanoparticles is adjacent to at least six other gold nanoparticles;
dissolving the compound in water to form a solution;
adding the first substrate at least partially coated with the gold nanoparticles to the solution to form a mixture;
wherein the gold nanoparticles are in a monolayer assembly on the substrate;
wherein the gold nanoparticles are 10-250 nm in size;
wherein a surface of the gold nanoparticles is not functionalized with an outer shell;
wherein the monolayer assembly is formed without a surfactant or linker;
removing the first substrate from the mixture and washing with water to form a SERS sample having at least a portion of molecules of the compound adsorbed to the gold nanoparticles on the first substrate; and
recording a SERS spectrum of the SERS sample;
wherein the SERS signal of the SERS spectrum is higher than a SERS signal of a SERS spectrum of the compound on a different substrate without the gold nanoparticles.