US 12,117,387 B2
Biosensor, method for manufacturing the same, and analysis method using the same
Hyun Chul Yoon, Seoul (KR); Jae-Ho Kim, Suwon-si (KR); and Ka Ram Kim, Suwon-si (KR)
Assigned to AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Suwon-si (KR)
Appl. No. 18/007,348
Filed by AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, Suwon-si (KR)
PCT Filed Jul. 28, 2021, PCT No. PCT/KR2021/009754
§ 371(c)(1), (2) Date Jan. 30, 2023,
PCT Pub. No. WO2022/025604, PCT Pub. Date Feb. 3, 2022.
Claims priority of application No. 10-2020-0095434 (KR), filed on Jul. 30, 2020.
Prior Publication US 2024/0255410 A1, Aug. 1, 2024
Int. Cl. G01N 21/47 (2006.01); G01N 15/1434 (2024.01); G01N 21/51 (2006.01); G01N 33/483 (2006.01)
CPC G01N 15/1434 (2013.01) [G01N 21/51 (2013.01); G01N 33/4833 (2013.01)] 13 Claims
OG exemplary drawing
 
1. An optical sensor comprising:
a first chamber having a first inner space receiving therein cells and a first culture medium;
a second chamber having a second inner space receiving therein a second culture medium and a chemoattractant, wherein the chemoattractant activates migration ability of the cells, wherein the second chamber is coupled to the first chamber so that a bottom of the first chamber is immersed in the second culture medium;
a light-emitter for irradiating light into an inside of the second chamber; and
a light-receiver configured to generate an optical image of light retroreflected from the inside of the second chamber, and to perform quantitative analysis of the retroreflected light based on the optical image,
wherein the first chamber includes:
the bottom having pores defined therein through which the cells can migrate into the second chamber; and
a coating layer disposed on the bottom, wherein the coating layer contains retroreflective particles therein, each having a size smaller than a size of each of the pores, wherein the retroreflective particles are capable of retroreflecting the light therefrom.