| CPC C12Q 1/6841 (2013.01) [C12N 15/1065 (2013.01)] | 40 Claims |
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1. A method of biological information generation, the method comprising:
providing node factories on a biological sample at node locations, wherein the node factories comprise one or more node nucleic acids, and wherein each node nucleic acid comprises:
a node barcode region;
one or more flanking node barcode hybridization regions;
a node nucleic acid restriction site; and
optionally, a node primer region;
amplifying the one or more node nucleic acids to generate concatemers of node nucleic acids, wherein the concatemers diffuse away from the node locations over time;
cleaving the concatemers into a plurality of node nucleic acids;
attaching two or more node nucleic acids generated by the node factories to generate multinode nucleic acids; and
analyzing for a frequency of association of the two or more node nucleic acids, wherein the frequency of association provides information for spatial mapping of the biological sample.
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